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SiRNA profiling of human primary prostate stromal cells knocked down for FOXF2

ABSTRACT: To identify the genes and pathways regulated by FOXF2, we investigated potential FOXF2 gene targets by microarray analyses of primary prostate stromal cells (PrSC) in which FOXF2 was knocked down by siRNA. 190 differentially expressed genes were selected, of which 104 genes were more highly expressed in PrSC cells treated with FOXF2 siRNA and 86 were more highly expressed in PRSC cells treated with negative control siRNA. Experiment Overall Design: In each experiment, we compared gene expression of PrSC cells treated with FOXF2 siRNA versus PrSC cells treated with negative control siRNA, in a total of 6 affymetrix arrays. 190 differentially expressed genes were selected (ratio negative control siRNA/siRNA â¥ 2log |0.8| as average in all arrays).

ORGANISM(S): Homo sapiens

SUBMITTER: Leonie van der Heul

PROVIDER: E-GEOD-12444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The FOXF2 pathway in the human prostate stroma.

van der Heul-Nieuwenhuijsen Leonie L Dits Natasja N Van Ijcken Wilfred W de Lange Don D Jenster Guido G

The Prostate 20091001 14

<h4>Background</h4>Forkhead box 2 (FOXF2) is a member of the large family of forkhead transcription factors and its expression pattern suggests a role in prostate cancer development. FOXF2 expression is stroma-specific and higher expressed in the prostate transition zone than the prostate peripheral zone. Moreover, expression of FOXF2 is decreased in prostate cancer.<h4>Methods</h4>To identify the genes and pathways regulated by FOXF2, we compared microarray expression profiles of primary prosta ...[more]

PMID: 19562724

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