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Trancriptomic profiling of HCT116 colon cancer cells with or without PBRM1 knock-down by RNAi

ABSTRACT: To investigate the influence of PBRM1 on gene expression, we performed RNA-seq in HCT116 colon cancer cells with or without PBRM1 knock-down by RNAi (both shRNA and siRNA approaches were used), and identified differentially expressed genes in PBRM1 knock-down cells compared to control cells, which shows implications for PBRM1's biological function in colon cancer.

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Homo sapiens

SUBMITTER: Xingsheng Shu

PROVIDER: E-MTAB-5566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The epigenetic modifier PBRM1 restricts the basal activity of the innate immune system by repressing retinoic acid-inducible gene-I-like receptor signalling and is a potential prognostic biomarker for colon cancer.

Shu Xing-Sheng XS Zhao Yingying Y Sun Yanmei Y Zhong Lan L Cheng Yingduan Y Zhang Yixiang Y Ning Kaile K Tao Qian Q Wang Yejun Y Ying Ying Y

The Journal of pathology 20171127 1

It has long been known that patients suffering from inflammatory bowel disease (IBD) have an increased risk of developing colorectal cancer (CRC). The innate immune system of host cells provides a first-line defence against pathogenic infection, whereas an uncontrolled inflammatory response under homeostatic conditions usually leads to pathological consequences, as exemplified by the chronic inflammation of IBD. The key molecules and pathways keeping innate immunity in check are still poorly def ...[more]

PMID: 28940253

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