Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of wheat development

ABSTRACT: The analysis of gene expression during wheat development:; Gene expression measurements were carried out on a developmental tissue; series for wild-type wheat (cv. Chinese Spring) using the Affymetrix; Wheat GeneChip. Thirteen tissues at defined developmental stages were; chosen to match the barley (cv. Morex) tissue series of Druka et al. 2006 that used the Affymetrix Barley1 GeneChip. Three replicates of:; root tissue at two different developmental stages, leaf, crown,; caryopsis, anther, pistil, inflorescence, bracts, mesocotyl, endosperm,; embryo and coleoptiles were hybridised. Comparisons between this wheat; data and the barley dataset were performed and are available at; http://contigcomp.acpfg.com.au ; [PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tim Sutton. The equivalent experiment is TA3 at PLEXdb.] Experiment Overall Design: tissue type: germinating seed, coleoptile(3-replications); tissue type: germinating seed, root(3-replications); tissue type: germinating seed, embryo(3-replications); tissue type: seedling, root(3-replications); tissue type: seedling, crown(3-replications); tissue type: seedling, leaf(3-replications); tissue type: immature inflorescence(3-replications); tissue type: floral bracts, before anthesis(3-replications); tissue type: pistil, before anthesis(3-replications); tissue type: anthers, before anthesis(3-replications); tissue type: 3-5 DAP caryopsis(3-replications); tissue type: 22 DAP embryo(3-replications); tissue type: 22 DAP endosperm(3-replications)

ORGANISM(S): Triticum aestivum

SUBMITTER: PLEXdb Curator Sudhansu Dash

PROVIDER: E-GEOD-12508 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comparative transcriptomics in the Triticeae.

Schreiber Andreas W AW Sutton Tim T Caldo Rico A RA Kalashyan Elena E Lovell Ben B Mayo Gwenda G Muehlbauer Gary J GJ Druka Arnis A Waugh Robbie R Wise Roger P RP Langridge Peter P Baumann Ute U

BMC genomics 20090629

<h4>Background</h4>Barley and particularly wheat are two grass species of immense agricultural importance. In spite of polyploidization events within the latter, studies have shown that genotypically and phenotypically these species are very closely related and, indeed, fertile hybrids can be created by interbreeding. The advent of two genome-scale Affymetrix GeneChips now allows studies of the comparison of their transcriptomes.<h4>Results</h4>We have used the Wheat GeneChip to create a "gene e ...[more]

PMID: 19558723

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Project description:Genome-wide gene responses in a transgenic rice line carrying the maize resistance gene Rxo1 to the rice bacterial streak pathogen, Xanthomonas oryzae pv. oryzicola; Non-host resistance in rice to its bacterial pathogen, Xanthomonas oryzae pv. oryzicola (Xoc), mediated by a maize NBS-LRR type R gene, Rxo1 shows a typical hypersensitive reaction (HR) phenotype, but the molecular mechanism(s) underlying this type of non-host resistance remain largely unknown. Results A microarray experiment was performed to reveal the molecular mechanisms underlying HR of rice to Xoc mediated by Rxo1 using a pair of transgenic and non-transgenic rice lines. Our results indicated that Rxo1 appeared to function in the very early step of the interaction between rice and Xoc, and could specifically activate large numbers of genes involved in signaling pathways leading to HR and some basal defensive pathways such as SA and ET pathways. In the former case, Rxo1 appeared to differ from the typical host R genes in that it could lead to HR without activating NDR1. In the latter cases, Rxo1 was able to induce a unique group of WRKY TF genes and a large set of genes encoding PPR and RRM proteins that share the same G-box in their promoter regions with possible functions in post-transcriptional regulation. In conclusion, Rxo1, like most host R genes, was able to trigger HR against Xoc in the heterologous rice plants by activating multiple defensive pathways related to HR, providing useful information on the evolution of plant resistance genes. Maize non-host resistance gene Rxo1 could trigger the pathogen-specific HR in heterologous rice, and ultimately leading to a localized programmed cell death which exhibits the characteristics consistent with those mediated by host resistance genes, but a number of genes encoding pentatricopeptide repeat and RNA recognition motif protein were found specifically up-regulated in the Rxo1 mediated disease resistance. These results add to our understanding the evolution of plant resistance genes. Experiment Overall Design: All inoculated flag leaves from three plants of 9804-Rxo1 and 9804 were collected two days post-inoculation (dpi), and the flag leaves from three non-inoculated check plants of 9804-Rxo1 and 9804 were also sampled at the same time point as control. The flag leaves from non-inoculated check plants of 9804 were sampled at the same time point as control: 9804-Rxo1 (3-replications), XOC - time: 2 dpi(3-replications); 9804-Rxo1 (3-replications); 9804, XOC - time: 2 dpi(3-replications) 9804. 2 sets of separately normalized data: 9804-Rxo1 and 9804.

Dataset Information

Transcription profiling of wheat development

Publications

Comparative transcriptomics in the Triticeae.

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