Unknown,Transcriptomics,Genomics,Proteomics

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Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines


ABSTRACT: We utilized the Barley1 Affymetrix GeneChip for comparative transcript analysis of Betzes barley, Chinese Spring wheat, and Chinese SpringM-bM-^@M-^SBetzes ditelosomic chromosome addition lines to physically map barley genes to their respective chromosome arm locations. We mapped barley genes to chromosome arms (1HS, 2HS, 2HL, 3HS, 3HL, 4HS, 4HL, 5HS, 5HL, 7HS, and 7HL) based on their transcript levels in the ditelosomic addition lines. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Hatice Bilgic. The equivalent experiment is BB55 at PLEXdb.] genotype: CS (Chinese Spring)(3-replications); genotype: Betzes(3-replications); genotype: 1HS(3-replications); genotype: 2HS(3-replications); genotype: 3HS(3-replications); genotype: 3HL(3-replications); genotype: 4HS(3-replications); genotype: 4HL(3-replications); genotype: 5HS(3-replications); genotype: 5HL(3-replications); genotype: 7HS(3-replications); genotype: 7HL(3-replications)

ORGANISM(S): Triticum aestivum

SUBMITTER: Hatice Bilgic 

PROVIDER: E-GEOD-33400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines.

Bilgic Hatice H   Cho Seungho S   Garvin David F DF   Muehlbauer Gary J GJ  

Genome 20071001 10


Wheat-barley disomic and ditelosomic chromosome addition lines have been used as genetic tools for a range of applications since their development in the 1980s. In the present study, we used the Affymetrix Barley1 GeneChip for comparative transcript analysis of the barley cultivar Betzes, the wheat cultivar Chinese Spring, and Chinese Spring - Betzes ditelosomic chromosome addition lines to physically map barley genes to their respective chromosome arm locations. We mapped 1257 barley genes to c  ...[more]

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