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TatA_vs_WT

ABSTRACT: Series of 4 repetitions of hybridization of treatment (tatA) and control (WT) each. Comparison of the Arabidopsis tata antisense line that is defective in the ion gradient dependent twin-arginine translocation (TAT) pathway for uptake from the stroma into, or passage through, the thylakoid membrane in the chloroplasts of higher plants versus WT. J. Marques, unpublished Keywords: repeat sample

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: christos Noutsos

PROVIDER: E-GEOD-1253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of 101 nuclear transcriptomes reveals 23 distinct regulons and their relationship to metabolism, chromosomal gene distribution and co-ordination of nuclear and plastid gene expression.

Biehl Alexander A Richly Erik E Noutsos Christos C Salamini Francesco F Leister Dario D

Gene 20050101

Post-endosymbiotic evolution of the proto-chloroplast was characterized by gene transfer to the nucleus. Hence, most chloroplast proteins are nuclear-encoded and the regulation of chloroplast functions includes nuclear transcriptional control. The expression profiles of 3292 nuclear Arabidopsis genes, most of them encoding chloroplast proteins, were determined from 101 different conditions and have been deposited at the GEO database (http://www.ncbi.nih.gov/geo/) under . The 1590 most-regulated ...[more]

PMID: 15656970

Similar Datasets

tatA_vs_WT

Project description:Series of 4 repetitions of hybridization of treatment (tatA) and control (WT) each. Comparison of the Arabidopsis tata antisense line that is defective in the ion gradient dependent twin-arginine translocation (TAT) pathway for uptake from the stroma into, or passage through, the thylakoid membrane in the chloroplasts of higher plants versus WT. J. Marques, unpublished Keywords: repeat sample

2005-01-01 | GSE1253 | GEO

Cell envelope adaptation and minimal Tat translocation are functionally intertwined

Project description:Protein secretion is key for bacterial growth, survival and adaptation. In the Gram-positive bacterium Bacillus subtilis, the twin-arginine (Tat) pathway serves in the exclusive secretion of proteins with bound cofactors. The Bacillus Tat pathway stands out for its minimalist nature with two independently acting translocases being composed of dedicated TatA and TatC subunits only. Here we addressed the question whether one of these translocases, TatAyCy, recruits additional common cellular components for activity. To this end, TatAyCy was purified by affinity chromatography and gel filtration, and interacting co-purified proteins were identified by mass spectrometry. This revealed the cell envelope stress responsive LiaH protein as an appendix to the TatAyCy complex. Importantly, our functional studies show that Tat expression is tightly trailed by LiaH induction, and that LiaH itself determines the capacity and quality of Tat-dependent protein translocation. Altogether, our observations show that protein translocation by the minimal Tat translocase TatAyCy is tightly intertwined with an adequate bacterial response to cell envelope stress. This is consistent with a critical need to maintain cellular homeostasis, especially when the membrane is widely opened to permit passage of large fully-folded proteins via Tat.

2022-08-11 | PXD012842 | Pride

The twin-arginine translocation system of Achromobacter xylosoxidans

Project description:Achromobacter xylosoxidans is an emerging pathogen, causing airway infections in patients with cystic fibrosis (CF). The knowledge about virulence factors and protein secretion systems in this bacterium is limited. The twin-arginine translocation (Tat) is a protein secretion system for the transportation of folded proteins across the inner cell membrane of Gram-negative bacteria. Here, we report a quantitative proteome analysis of fractionated Achromobacter xylosoxidans wt and Tat-mutant cells in iron-containing and iron-lacking conditions.

2023-06-15 | MSV000092175 | MassIVE

Proteomics investigation of the time course responses of RAW264.7 macrophages to infections with the wild-type and twin-arginine translocation mutant strains of Brucella melitensis

Project description:Brucella, a notorious intracellular pathogen, causes chronic infections in many mammals, including humans. The twin-arginine translocation (Tat) pathway transports folded proteins across the cytoplasmic membrane; protein substrates translocated by Brucella include ABC transporters, oxidoreductases, and cell envelope biosynthesis proteins. Previously, we showed that a Tat mutant of Brucella melitensis M28 exhibits reduced survival within murine macrophages. In this study, we compared the host responses elicited by wild-type M28 and its Tat-mutant strains ex vivo. We utilized label-free quantitative proteomics to assess proteomic changes in RAW264.7 macrophages after infection with M28 and its Tat mutants.

2021-07-05 | PXD024658 | Pride

Dynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway

Project description:In Escherichia coli, the Twin-arginine (Tat) secretion system is one of the main routes of the protein export to the periplasm. The Tat pathway secretes a set of proteins with important physiological functions. In our study, we investigated the influence of the deactivation of the Tat pathway on the E. coli cells. We applied a comprehensive and comparative proteomic analysis of the E. coli wild type and tat mutant. This dataset provides mass spectrometry based details on the abundances of proteins in cytoplasmic, periplasmic and membrane fractions. We observed that a tat deletion increases abundances of proteins involved in protein folding, degradation, responses to heat, oxidation, osmolarity, and cold. Moreover, the impairment of E. coli outer membrane resulted in the activation of proteins responsible for cell wall biogenesis. The tat deletion negatively affects the synthesis of iron transporters and imbalances its homeostasis in the cell.

2019-02-18 | PXD008803 | Pride

prors1_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (prors1) and control (WT) each. Comparison of the Arabidopsis prors1 mutant lacking prolyl tRNA synthetase versus WT. P. Pesaresi, D. Leister, unpublished Keywords: repeat sample

2004-12-31 | E-GEOD-1232 | biostudies-arrayexpress

prors2_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (prors2) and control (WT) each. Comparison of the Arabidopsis prors2 mutant lacking prolyl tRNA synthetase versus WT. P. Pesaresi, D. Leister, unpublished Keywords: repeat sample

2004-12-31 | E-GEOD-1233 | biostudies-arrayexpress

psae2_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (psae2) and control (WT) each. Comparison of the Arabidopsis psae2 mutant lacking Photosystem I protein PSI-E2 versus WT. A. Ihnatowicz, D. Leister, unpublished Keywords: repeat sample

2004-12-31 | E-GEOD-1236 | biostudies-arrayexpress

psan_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (psan) and control (WT) each. Comparison of the Arabidopsis psan mutant lacking Photosystem I protein PSI-N versus WT. A. Dietzmann, D. Leister, unpublished Keywords: repeat sample

2010-06-10 | E-GEOD-1243 | biostudies-arrayexpress

psao_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (psao) and control (WT) each. Comparison of the Arabidopsis psao mutant lacking Photosystem I protein PSI-O versus WT. A. Dietzmann, D. Leister, unpublished Keywords: repeat sample

2010-06-10 | E-GEOD-1244 | biostudies-arrayexpress

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