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TatA_vs_WT

ABSTRACT: Series of 4 repetitions of hybridization of treatment (tatA) and control (WT) each. Comparison of the Arabidopsis tata antisense line that is defective in the ion gradient dependent twin-arginine translocation (TAT) pathway for uptake from the stroma into, or passage through, the thylakoid membrane in the chloroplasts of higher plants versus WT. J. Marques, unpublished Keywords: repeat sample

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE1253 | GEO | 2005/01/01

SECONDARY ACCESSION(S): PRJNA87483

REPOSITORIES: GEO

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tatA_vs_WT

Project description:Series of 4 repetitions of hybridization of treatment (tatA) and control (WT) each. Comparison of the Arabidopsis tata antisense line that is defective in the ion gradient dependent twin-arginine translocation (TAT) pathway for uptake from the stroma into, or passage through, the thylakoid membrane in the chloroplasts of higher plants versus WT. J. Marques, unpublished Keywords: repeat sample

2010-06-21 | E-GEOD-1253 | biostudies-arrayexpress

Cell envelope adaptation and minimal Tat translocation are functionally intertwined

Project description:Protein secretion is key for bacterial growth, survival and adaptation. In the Gram-positive bacterium Bacillus subtilis, the twin-arginine (Tat) pathway serves in the exclusive secretion of proteins with bound cofactors. The Bacillus Tat pathway stands out for its minimalist nature with two independently acting translocases being composed of dedicated TatA and TatC subunits only. Here we addressed the question whether one of these translocases, TatAyCy, recruits additional common cellular components for activity. To this end, TatAyCy was purified by affinity chromatography and gel filtration, and interacting co-purified proteins were identified by mass spectrometry. This revealed the cell envelope stress responsive LiaH protein as an appendix to the TatAyCy complex. Importantly, our functional studies show that Tat expression is tightly trailed by LiaH induction, and that LiaH itself determines the capacity and quality of Tat-dependent protein translocation. Altogether, our observations show that protein translocation by the minimal Tat translocase TatAyCy is tightly intertwined with an adequate bacterial response to cell envelope stress. This is consistent with a critical need to maintain cellular homeostasis, especially when the membrane is widely opened to permit passage of large fully-folded proteins via Tat.

2022-08-11 | PXD012842 | Pride

prors2_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (prors2) and control (WT) each. Comparison of the Arabidopsis prors2 mutant lacking prolyl tRNA synthetase versus WT. P. Pesaresi, D. Leister, unpublished Keywords: repeat sample

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Project description:Series of 6 repetitions of hybridization of treatment (prors1) and control (WT) each. Comparison of the Arabidopsis prors1 mutant lacking prolyl tRNA synthetase versus WT. P. Pesaresi, D. Leister, unpublished Keywords: repeat sample

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psae2_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (psae2) and control (WT) each. Comparison of the Arabidopsis psae2 mutant lacking Photosystem I protein PSI-E2 versus WT. A. Ihnatowicz, D. Leister, unpublished Keywords: repeat sample

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Project description:Series of 6 repetitions of hybridization of treatment (psao) and control (WT) each. Comparison of the Arabidopsis psao mutant lacking Photosystem I protein PSI-O versus WT. A. Dietzmann, D. Leister, unpublished Keywords: repeat sample

2005-01-01 | GSE1244 | GEO

psan_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (psan) and control (WT) each. Comparison of the Arabidopsis psan mutant lacking Photosystem I protein PSI-N versus WT. A. Dietzmann, D. Leister, unpublished Keywords: repeat sample

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toc132_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (toc132) and control (WT) each. Comparison of the Arabidopsis toc132 mutant exhibiting an impaired protein import across the outer chloroplast envelope vs WT. P.Jarvis, unpublished Keywords: repeat sample

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toc75_vs_WT

Project description:Series of 6 repetitions of hybridization of treatment (toc75) and control (WT) each. Comparison of the Arabidopsis toc75 mutant exhibiting an impaired protein import across the outer chloroplast envelope vs WT. P. Jarvis, unpublished Keywords: repeat sample

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Project description:Series of 6 repetitions of hybridization of treatment (toc160) and control (WT) each. Comparison of the Arabidopsis toc160 mutant exhibiting an impaired protein import across the outer chloroplast envelope vs WT. P. Jarvis, unpublished Keywords: repeat sample

2005-01-01 | GSE1257 | GEO

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