Profiles and ChIP on chip experiments with dl1500 virus (expressing WT small e1a), R2G and deltaCR2 mutant viruses

ABSTRACT: Adenovirus e1a induces quiescent human cells to divide. We found that e1a causes global relocalization of the RB-proteins (RB, p130, p107) and p300/CBP histone acetyltransferases on promoters that restricts H3K18ac to a limited set of genes to stimulate cell cycling and inhibit antiviral responses and cellular differentiation. Soon after expression, e1a binds transiently to cell cycle/growth genes, causing enrichment of p300/CBP, PCAF, H3K18ac, depletion of RB-proteins, and transcriptional activation. e1a also associates transiently with antiviral genes, causing enrichment for RB, p130, H4K16ac, increased nucleosome density, and repression. At later times, e1a and p107 bind mainly to development/differentiation genes, repressing transcription. The temporal order of e1a binding required its interactions with p300/CBP and RB-proteins. Our data uncover a defined transcriptional and epigenetic reprogramming leading to cellular transformation. This SuperSeries is composed of the following subset Series: GSE12045: Expression profiles and ChIP on chip genomewide experiments with dl1500 virus (expressing WT small e1a). GSE12046: Expression profiles and ChIP on chip genomewide experiments with R2G mutant virus GSE12047: Expression profiles and ChIP on chip genomewide experiments with deltaCR2 mutant virus Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Siavash Kurdistani 

PROVIDER: E-GEOD-12543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress