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Gene expression profiling of maize anther and pollen ontogeny


ABSTRACT: Global gene expression profiles of seven stages representing 29 days of anther development are analyzed using a 44K oligonucleotide array querying ~80% of maize protein-coding genes. Each anther stage expresses ~10,000 constitutive and ~10,000 or more transcripts restricted to one or a few stages. Keywords: anther development, maize 4 replicates of 7 samples (6 anther stages plus mature pollen) were hybridized to a 44K Agilent array according to the A-optimal design recommended by Kerr and Churchill for 7 samples.

ORGANISM(S): Zea mays

SUBMITTER: John Fernandes 

PROVIDER: E-GEOD-12579 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Male reproductive development: gene expression profiling of maize anther and pollen ontogeny.

Ma Jiong J   Skibbe David S DS   Fernandes John J   Walbot Virginia V  

Genome biology 20081219 12


<h4>Background</h4>During flowering, central anther cells switch from mitosis to meiosis, ultimately forming pollen containing haploid sperm. Four rings of surrounding somatic cells differentiate to support first meiosis and later pollen dispersal. Synchronous development of many anthers per tassel and within each anther facilitates dissection of carefully staged maize anthers for transcriptome profiling.<h4>Results</h4>Global gene expression profiles of 7 stages representing 29 days of anther d  ...[more]

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