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Divergent transcription from active promoters

ABSTRACT: Here we describe a novel class of short RNAs and high-resolution ChIP-Seq data that together indicate widespread divergent transcription at mammalian promoters and suggest the existence of novel regulatory mechanisms concerning both initiation and elongation by RNA polymerase II. ChIP-seq in murine embryonic stem cells for RNA polymerase II

ORGANISM(S): Mus musculus

SUBMITTER: Stuart Levine

PROVIDER: E-GEOD-12680 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Divergent transcription from active promoters.

Seila Amy C AC Calabrese J Mauro JM Levine Stuart S SS Yeo Gene W GW Rahl Peter B PB Flynn Ryan A RA Young Richard A RA Sharp Phillip A PA

Science (New York, N.Y.) 20081204 5909

Transcription initiation by RNA polymerase II (RNAPII) is thought to occur unidirectionally from most genes. Here, we present evidence of widespread divergent transcription at protein-encoding gene promoters. Transcription start site-associated RNAs (TSSa-RNAs) nonrandomly flank active promoters, with peaks of antisense and sense short RNAs at 250 nucleotides upstream and 50 nucleotides downstream of TSSs, respectively. Northern analysis shows that TSSa-RNAs are subsets of an RNA population 20 t ...[more]

PMID: 19056940

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