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Transcription profiling of human growth plate chrondrocytes grown in monolayer, 0.5 vs. 20% serum

ABSTRACT: Human growth plate chondrocytes were isolated from the distal femoral growth plate from a single adolescent undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term. Cells were replated and cultured in 0.5% or 20% serum.

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Olney

PROVIDER: E-GEOD-1277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Growth factor regulation of human growth plate chondrocyte proliferation in vitro.

Olney Robert C RC Wang Jianwei J Sylvester James E JE Mougey Edward B EB

Biochemical and biophysical research communications 20040501 4

Linear growth occurs as the result of growth plate chondrocytes undergoing proliferative and hypertrophic phases. Paracrine feedback loops that regulate the entry of chondrocytes into the hypertrophic phase have been shown and similar pathways likely exist for the proliferative phase. Human long-bone growth plate chondrocytes were cultured in vitro. The proliferative effects of a variety of factors were determined by [3H]thymidine uptake and the gene expression profile of these cells was determi ...[more]

PMID: 15094393

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HGPC, in monolayer, 0.5 vs. 20% serum

Project description:Human growth plate chondrocytes were isolated from the distal femoral growth plate from a single adolescent undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term. Cells were replated and cultured in 0.5% or 20% serum. Keywords: parallel sample

2004-04-13 | GSE1277 | GEO

HGPC, in monolayer, 0.5% serum

Project description:Human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term, in 20% serum. Cells were replated and cultured 48 h in 0.5% serum. Keywords: repeat sample

2004-04-13 | GSE1278 | GEO

Transcription profiling of human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis

2008-06-12 | E-GEOD-1278 | biostudies-arrayexpress

Gene Expression Across the Human Growth Plate

Project description:Chondrocytes advance through the long bone growth plate in synchrony, allowing their segregation into one of four zones each corresponding to a different phase of maturation. Beginning from the epiphysis the recognized zones are: reserve, proliferative, prehypertrophic, and hypertrophic. A fifth zone, the perichondrium is located in apposition to the growth plate at its circumferential surface. The progression of chondrocytes through these zones, and hence growth, is regulated by a complex interplay of signaling pathways, defects in which lead to osteochondrodysplasias. At present, there are over 370 recognized skeletal disorders, slightly more than half of which have been explained at the molecular level. Through a combination of laser microdissection, linear mRNA amplification, and GeneChip analysis, our laboratory has acquired the zone specific expression profile of chondrocytes from the distal femoral growth plates of two normal patients. On average, transcripts interrogated by 12,193 or 18,454 GeneChip probe sets (out of a possible 54,000 probe sets) were present in patients 1 and 2, respectively. The homologous inter-array correlations for the log(2) intensities were M-bM-^IM-% 0.91M-BM-10.01. Keywords: Chondrocyte differentiation program. Populations of cells corresponding to the hypertrophic, prehypertrophic, proliferative, reserve, and perichondrium, were separately isolated by laser microdissection from cryostat sections of human growth plates using a Leica AS-LMD microscope. RNA was prepared from the isolated cells, amplified using a T7 linear amplification protocol, and analyzed by hybridization to Affymetrix microarrays. The distal femoral growth plate (left or right) was exclusively used for this study. Patient 1 of this study is an 11-10/12 year old Caucasian female and patient 2 is a 13-3/12 year old Caucasian male.

2013-09-13 | E-GEOD-9160 | biostudies-arrayexpress

Gene Expression Across the Human Growth Plate

2013-09-13 | GSE9160 | GEO

Hnrnpk orchestrates embryonic growth plate development via regulating glycolysis

Project description:Here we show a multifunctional protein, Hnrnpk, is essential for preventing excessive apoptosis and premature differentiation of growth plate chondrocytes. These datas were the RNA-seq of E18.5 growth plate chondrocytes with or without Hnrnpk.

2022-09-28 | GSE197283 | GEO

Gene expression profile at single vell level of growth plate chondrocytes [scRNA-seq]

Project description:Gowth plate chondrocytes include heterogenous cell population and cellular diversity of these chondrocytes remains to be elucidated. We used single cell RNA sequencing to uncover the diverssity of growth plate chondrocytes and identify noel marker genes selectively expressed in pre-hypertrophic chondrocytes.

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HGPC, in monolayer, 0.5 vs. 20% serum, complete

Project description:This SuperSeries is composed of the SubSeries listed below.

2006-12-03 | GSE6429 | GEO

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Gene expression profiling of articular and growth plate cartilages

Project description:To identify genes that maintain the homeostasis of the articular cartilage, we compared gene expression profiles of adult articular cartilage chondrocytes with that of growth plate cartilage chondrocytes in adult (10-week-old) Sprague Dawley (SD) rats. Furthermore, to identify genes that have a potency to regenerate the articular cartilage, we compared gene expression profiles of superficial layer chondrocytes of infant epiphyseal cartilage which form articular cartilage with that of the deep layer chondrocytes which form growth plate cartilage in infant (6-day-old) SD rats.

2012-07-17 | E-MTAB-1218 | biostudies-arrayexpress

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