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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human growth plate chrondrocytes grown in monolayer, 0.5 vs. 20% serum

ABSTRACT: Human growth plate chondrocytes were isolated from the distal femoral growth plate from a single adolescent undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term. Cells were replated and cultured in 0.5% or 20% serum.

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Olney

PROVIDER: E-GEOD-1277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Growth factor regulation of human growth plate chondrocyte proliferation in vitro.

Olney Robert C RC Wang Jianwei J Sylvester James E JE Mougey Edward B EB

Biochemical and biophysical research communications 20040501 4

Linear growth occurs as the result of growth plate chondrocytes undergoing proliferative and hypertrophic phases. Paracrine feedback loops that regulate the entry of chondrocytes into the hypertrophic phase have been shown and similar pathways likely exist for the proliferative phase. Human long-bone growth plate chondrocytes were cultured in vitro. The proliferative effects of a variety of factors were determined by [3H]thymidine uptake and the gene expression profile of these cells was determi ...[more]

PMID: 15094393

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HGPC, in monolayer, 0.5 vs. 20% serum

Project description:Human growth plate chondrocytes were isolated from the distal femoral growth plate from a single adolescent undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term. Cells were replated and cultured in 0.5% or 20% serum. Keywords: parallel sample

2004-04-13 | GSE1277 | GEO

HGPC, in monolayer, 0.5% serum

Project description:Human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term, in 20% serum. Cells were replated and cultured 48 h in 0.5% serum. Keywords: repeat sample

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Transcription profiling of human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis

2008-06-12 | E-GEOD-1278 | biostudies-arrayexpress

Gene Expression Across the Human Growth Plate

Project description:Chondrocytes advance through the long bone growth plate in synchrony, allowing their segregation into one of four zones each corresponding to a different phase of maturation. Beginning from the epiphysis the recognized zones are: reserve, proliferative, prehypertrophic, and hypertrophic. A fifth zone, the perichondrium is located in apposition to the growth plate at its circumferential surface. The progression of chondrocytes through these zones, and hence growth, is regulated by a complex interplay of signaling pathways, defects in which lead to osteochondrodysplasias. At present, there are over 370 recognized skeletal disorders, slightly more than half of which have been explained at the molecular level. Through a combination of laser microdissection, linear mRNA amplification, and GeneChip analysis, our laboratory has acquired the zone specific expression profile of chondrocytes from the distal femoral growth plates of two normal patients. On average, transcripts interrogated by 12,193 or 18,454 GeneChip probe sets (out of a possible 54,000 probe sets) were present in patients 1 and 2, respectively. The homologous inter-array correlations for the log(2) intensities were M-bM-^IM-% 0.91M-BM-10.01. Keywords: Chondrocyte differentiation program. Populations of cells corresponding to the hypertrophic, prehypertrophic, proliferative, reserve, and perichondrium, were separately isolated by laser microdissection from cryostat sections of human growth plates using a Leica AS-LMD microscope. RNA was prepared from the isolated cells, amplified using a T7 linear amplification protocol, and analyzed by hybridization to Affymetrix microarrays. The distal femoral growth plate (left or right) was exclusively used for this study. Patient 1 of this study is an 11-10/12 year old Caucasian female and patient 2 is a 13-3/12 year old Caucasian male.

2013-09-13 | E-GEOD-9160 | biostudies-arrayexpress

Gene Expression Across the Human Growth Plate

2013-09-13 | GSE9160 | GEO

Hnrnpk orchestrates embryonic growth plate development via regulating glycolysis

Project description:Here we show a multifunctional protein, Hnrnpk, is essential for preventing excessive apoptosis and premature differentiation of growth plate chondrocytes. These datas were the RNA-seq of E18.5 growth plate chondrocytes with or without Hnrnpk.

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HGPC, in monolayer, 0.5 vs. 20% serum, complete

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2006-12-03 | GSE6429 | GEO

Single cell RNAseq analysis of Col2a1+ growth plate chondrocytes

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2012-07-17 | E-MTAB-1218 | biostudies-arrayexpress

Bovine Hypertrophic Growth Plate Chondrocytes vs. Reserve Zone Chondrocytes

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