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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis

ABSTRACT: Human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term, in 20% serum. Cells were replated and cultured 48 h in 0.5% serum.

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Olney

PROVIDER: E-GEOD-1278 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Growth factor regulation of human growth plate chondrocyte proliferation in vitro.

Olney Robert C RC Wang Jianwei J Sylvester James E JE Mougey Edward B EB

Biochemical and biophysical research communications 20040501 4

Linear growth occurs as the result of growth plate chondrocytes undergoing proliferative and hypertrophic phases. Paracrine feedback loops that regulate the entry of chondrocytes into the hypertrophic phase have been shown and similar pathways likely exist for the proliferative phase. Human long-bone growth plate chondrocytes were cultured in vitro. The proliferative effects of a variety of factors were determined by [3H]thymidine uptake and the gene expression profile of these cells was determi ...[more]

PMID: 15094393

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HGPC, in monolayer, 0.5% serum

Project description:Human growth plate chondrocytes were isolated from the distal femoral growth plate from 3 different adolescents undergoing epiphysiodesis. Cell were isolated and grown in monolayers, long term, in 20% serum. Cells were replated and cultured 48 h in 0.5% serum. Keywords: repeat sample

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Transcription profiling of human growth plate chrondrocytes grown in monolayer, 0.5 vs. 20% serum

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Rat diaphyseal femoral fracture healing: Plate fixation vs. intramedullary nail

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Single cell RNAseq analysis of Col2a1+ growth plate chondrocytes

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Project description:Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. 6 pairs of HZ vs RZ were compared by microarray. Experiment Overall Design: Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. Six independent sample pairs of HZ vs RZ were compared by microarray.

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Gene Expression Across the Human Growth Plate

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2013-09-13 | E-GEOD-9160 | biostudies-arrayexpress

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