Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Genes upregulated by HLX

ABSTRACT: HLX was found as a VEGF-A induced gene in HUVEC (B.Schweighofer, submitted). In order to detect genes regulated by HLX HUVEC were infected by recombinant adenovirus expressing HLX for 4, 8, 16 and 32h. RNA was isolated and subjected to microarray analysis using Affymetrix microarray. HUVEC were infected with a MOI of 40 with either HLX expressing or empty adenovirus for 4, 8, 16 or 32 hours. HUVEC without an adenovirus infection were used as control.Total RNA was isolated with Trizol according to the instructions of the manufacturer. Gene expression was analysed with Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays according to the Affymetrix protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Julia Testori 

PROVIDER: E-GEOD-13054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

The VEGF-regulated transcription factor HLX controls the expression of guidance cues and negatively regulates sprouting of endothelial cells.

Testori Julia J   Schweighofer Bernhard B   Helfrich Iris I   Sturtzel Caterina C   Lipnik Karoline K   Gesierich Sabine S   Nasarre Patrick P   Hofer-Warbinek Renate R   Bilban Martin M   Augustin Hellmut G HG   Hofer Erhard E  

Blood 20110111 9

The HLX gene encoding a diverged homeobox transcription factor has been found to be up-regulated by vascular endothelial growth factor-A (VEGF-A) in endothelial cells. We have now investigated the gene repertoire induced by HLX and its potential biologic function. HLX strongly increased the transcripts for several repulsive cell-guidance proteins including UNC5B, plexin-A1, and semaphorin-3G. In addition, genes for transcriptional repressors such as HES-1 were up-regulated. In line with these fi  ...[more]

Similar Datasets

Transcriptomics Genes upregulated by HLX
2011-02-15 | GSE13054 | GEO
Transcriptomics Egr-1 induced gene expression changes in HUVECs
2005-12-01 | GSE2299 | GEO
Transcriptomics Transcription profiling of HUVEC infected with recombinant adenoviruses expressing EGR-1 or empty control viruses at a MOI of 100. RNA was isolated from the cells at the time points 16 h, 24 h and 48 h post infection
2007-10-27 | E-GEOD-2299 | biostudies-arrayexpress
Transcriptomics Mock or KSHV cells (BJABJ, BCBL, TIME and HUVEC) vs common reference (mixture of RNA from both infected and uninfected cells)
2010-07-07 | GSE21972 | GEO
Transcriptomics Sox18 regulated genes
2012-01-23 | GSE8952 | GEO
Transcriptomics Sox18 regulated genes
2012-01-23 | E-GEOD-8952 | biostudies-arrayexpress
Transcriptomics Activated Notch-induced transcriptional profile modulation in human primary dermal lymphatic endothelial cells
2010-03-20 | E-GEOD-20978 | biostudies-arrayexpress
Transcriptomics Mock or KSHV cells (BJABJ, BCBL, TIME and HUVEC) vs common reference (mixture of RNA from both infected and uninfected cells)
2010-07-07 | E-GEOD-21972 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human embryonic kidney cell line HEK293 infected with Adenovirus expressing cDNA for GFP or PTTG.
2009-12-31 | E-MEXP-2449 | biostudies-arrayexpress
Proteomics Immunoprecipitaiton of Rab27a from mouse melanoma cells
2020-05-15 | PXD018083 | Pride