Unknown,Transcriptomics,Genomics,Proteomics

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Mock or KSHV cells (BJABJ, BCBL, TIME and HUVEC) vs common reference (mixture of RNA from both infected and uninfected cells)


ABSTRACT: Expression profiling of latently infected cells using a custom tiling microarray HUVEC and TIME cells were infected BCBL-1-derived KSHV. Mock infected HUVEC and TIME cells served as controls for each of these two stably infected cells, respectively. BJAB cells served as uninfected controls for the BCBL-1 cells. KSHV-infected cells are induced to enter lytic cycle with valproate or Adenovirus-RTA. Cells were harvested at indicated time points and analyzed. Three condition experiment: mock infected, latently infected cells and lytically infected. Three cell types (BJAB cells served as uninfected controls for the BCBL-1 cells).

ORGANISM(S): Homo sapiens

SUBMITTER: Sanjay Chandriani 

PROVIDER: E-GEOD-21972 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The lytic transcriptome of Kaposi's sarcoma-associated herpesvirus reveals extensive transcription of noncoding regions, including regions antisense to important genes.

Chandriani Sanjay S   Xu Yiyang Y   Ganem Don D  

Journal of virology 20100609 16


Genomewide analyses of the mammalian transcriptome have revealed that large tracts of sequence previously annotated as noncoding are frequently transcribed and give rise to stable RNA. Although the transcription of individual genes of the Kaposi's sarcoma-associated herpesvirus (KSHV) has been well studied, little is known of the architecture of the viral transcriptome on a genomewide scale. Here we have employed a genomewide tiling array to examine the lytic transcriptome of the Kaposi's sarcom  ...[more]

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