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The effects of temporally restricted feeding on hepatic gene expression of Cry1, Cry2 double KO mice

ABSTRACT: Restricted feeding impacts the hepatic circadian clock of WT mice. Cry1, Cry2 double KO mice lack a circadian clock and are thus expected to show rhythmical gene expression in the liver. Imposing a temporally restricted feeding schedule on these mice shows how the hepatic circadian clock and rhythmic food intake regulate rhythmic transcription in parallel Cry1, Cry2 double KO mice were entrained either to ad libitum or temporally restricted feeding (tRF) schedules. Food was made available to mice under the tRF regimen only between ZT(CT)1 and ZT(CT)9. Mice were then released into constant darkness while the respective feeding schedules were still maintained. Liver tissue was collected on the second day of constant darkness at the indicated timepoints. Total RNA was extracted and 5ug of RNA was used in the standard Affymetrix protocol for amplification, labeling and hybridization

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Vollmers 

PROVIDER: E-GEOD-13062 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Time of feeding and the intrinsic circadian clock drive rhythms in hepatic gene expression.

Vollmers Christopher C   Gill Shubhroz S   DiTacchio Luciano L   Pulivarthy Sandhya R SR   Le Hiep D HD   Panda Satchidananda S  

Proceedings of the National Academy of Sciences of the United States of America 20091125 50

In mammals, the circadian oscillator generates approximately 24-h rhythms in feeding behavior, even under constant environmental conditions. Livers of mice held under constant darkness exhibit circadian rhythm in abundance in up to 15% of expressed transcripts. Therefore, oscillations in hepatic transcripts could be driven by rhythmic food intake or sustained by the hepatic circadian oscillator, or a combination of both. To address this question, we used distinct feeding and fasting paradigms on  ...[more]

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