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The effects of temporally restricted feeding on hepatic gene expression

ABSTRACT: Temporally restricted feeding is known to impact the circadian clock. This dataset shows the effects of temporally restricted feeding on the hepatic transcriptome. C57/B6 mice were entrained for two weeks to a temporally restricted feeding schedule. Food was made available only between ZT(CT)1 and ZT(CT)9. Mice were then released into constant darkness while food availability was still restricted and liver tissue was collected at the indicated timepoints on the second day in constant darkness. Total RNA was extracted and 5ug were submitted to the standard Affymetrix protocol for amplification, labeling and hybridization.

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Vollmers

PROVIDER: E-GEOD-13060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Time of feeding and the intrinsic circadian clock drive rhythms in hepatic gene expression.

Vollmers Christopher C Gill Shubhroz S DiTacchio Luciano L Pulivarthy Sandhya R SR Le Hiep D HD Panda Satchidananda S

Proceedings of the National Academy of Sciences of the United States of America 20091125 50

In mammals, the circadian oscillator generates approximately 24-h rhythms in feeding behavior, even under constant environmental conditions. Livers of mice held under constant darkness exhibit circadian rhythm in abundance in up to 15% of expressed transcripts. Therefore, oscillations in hepatic transcripts could be driven by rhythmic food intake or sustained by the hepatic circadian oscillator, or a combination of both. To address this question, we used distinct feeding and fasting paradigms on ...[more]

PMID: 19940241

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