Unknown,Transcriptomics,Genomics,Proteomics

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Arabidopsis unfertilized pistil development and senescence

ABSTRACT: Transcription profiling of post-anthesis unfertilized pistil development and senescence in Arabidopsis, in a serie including samples from seven different stages according to pistil age in days post-anthesis (dpa). The time course started at anthesis and ended at 12-14 dpa, several days later of the loss of fruit set capacity. Keywords: Developmental time course Unfertilized pistils were obtained growing under low humidity cer6-2 conditional male sterile mutant of Arabidopsis in Ler background. The seven stages considered were: 0-1 dpa, 2-3 dpa, 4-5 dpa, 6-7 dpa, 8-9 dpa 10-11 dpa and 12-14 dpa. Two biological replicas with two technical replicas each were performed for each stage. The biological replicas were grown independently, and all the samples of each one were harvested simultaneously. Amplified RNA from each sample was hibridized together with a global reference over the DNA microarray. The reference was generated from an equimolar mix of amplified RNAs from each of the seven samples of the corresponding biological replica. Amplified RNA from each sample was labelled separately with Cy5 and with Cy3, and each labelling was compared in the DNA microarray with Cy3-labelled and with Cy5-labelled RNA from the reference mix, respectively.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Pablo Carbonell

PROVIDER: E-GEOD-13113 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Copper and iron are essential micronutrients for most living organisms because they participate as cofactors in biological processes including respiration, photosynthesis and oxidative stress protection. In many eukaryotic organisms, including yeast and mammals, copper and iron homeostases are highly interconnected; however such interdependence is not well established in higher plants. Here we propose that COPT2, a high-affinity copper transport protein, functions under copper and iron deficiencies in Arabidopsis thaliana. COPT2 is a plasma membrane protein that functions in copper acquisition and distribution. Characterization of the COPT2 expression pattern indicates a synergic response to copper and iron limitation in roots. We have characterized a knockout of COPT2, copt2-1, that leads to increased resistance to simultaneous copper and iron deficiencies, measured as reduced leaf chlorosis and improved maintenance of the photosynthetic apparatus. We propose that COPT2 expression could play a dual role under Fe deficiency. First, COPT2 participates in the attenuation of copper deficiency responses driven by iron limitation maybe aimed to minimize further iron consume. On the other hand, global expression analyses of copt2-1 mutants versus wild type Arabidopsis plants indicate that low phosphate responses are increased in copt2-1 plants. In this sense, COPT2 function under Fe deficiency counteracts low phosphate responses. These results open up new biotechnological approaches to fight iron deficiency in crops. Four biological replicates of Arabidopsis seedlings were generated for 2 genotypes, Col-0 and copt2-1 mutant; and 3 growth condictions; first one an iron(Fe) and copper(Cu) sufficient medium (+Fe + Cu), second one an Fe deficient and Cu sufficient medium (-Fe+Cu) and third one an Fe and Cu deficient medium (-Fe-Cu). For each growth one comparasion was made, copt2-1 mutant versus Col-0; in each comparasion four biological replicates were made, two replicas were labeled with Cy5 for the mutant sample and Cy3 for the Col-0 sample, while the other two replicas were reversed-labeled.

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Arabidopsis unfertilized pistil development and senescence

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