Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Effect of feeding and females on male salivary glands

ABSTRACT: A differential display experiment was done to compare genes expressed in salivary glands from unfed males, males fed in the presence of females and males fed in the absence of females. Possible differential bands were excised, cloned and sequenced. A macroarray study was done on these clones as probes. RNA from the salivary glands of ticks from the three conditions was labelled and hybridized. Two sets of primers were used in the differential display, and these same two were used in the labelling, so there are two platforms, corresponding to what primer pair was used. Two independent RNA isolations were done, Experiment 1 used one, 2 and 3 the other. Experiment 2 used exposure to X-ray film, while 1 and 3 used a phosphorimager plate. Two exposures of the X-ray film were found to give acceptable results, 1 and 5 min. These were averaged in the analysis. Keywords = salivary_gland Keywords = feeding Keywords = male Keywords = female

ORGANISM(S): Dermacentor andersoni

SUBMITTER: Richard Essenberg

PROVIDER: E-GEOD-1320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression in male tick salivary glands is affected by feeding in the presence of females.

Anyomi Felix M FM Bior Abdelaziz D AD Essenberg Richard C RC Sauer John R JR

Archives of insect biochemistry and physiology 20061201 4

In this study, we have compared gene expression in the salivary glands of male Dermacentor andersoni ticks fed in the presence or absence of females to that in unfed males. We have established that the patterns of expression are different using arbitrarily primed polymerase chain reaction following reverse transcription of RNA (RAP-PCR) and differential display. Our results indicate a significant difference in salivary gland gene expression between fed and unfed males and also between males fed ...[more]

PMID: 17103403

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Dataset Information

Effect of feeding and females on male salivary glands

Publications

Gene expression in male tick salivary glands is affected by feeding in the presence of females.

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