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Splenic CD8+ and CD8- DCs

ABSTRACT: Analysis of gene expressions in mouse splenic dendritic cells (DCs). DCs were purified into two subsets, CD8-positive and -negative ones. DCs were expanded in vivo by injecting Flt3L-producing tumors into the backs of C57BL/6 mice. To expand DCs in vivo, Flt3L-producing B16 melanoma cells were injected to the backs of mice. Then, 10-12 days later, splenic DCs were enriched by MACS and purified into CD3-B220-CD8a+CD11c+ and CD3-B220-CD8a-CD11c+ cells by FACS cell sorter.

ORGANISM(S): Mus musculus

SUBMITTER: Hiroaki Hemmi 

PROVIDER: E-GEOD-13250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.

Hemmi Hiroaki H   Idoyaga Juliana J   Suda Koji K   Suda Nao N   Kennedy Kathleen K   Noda Masaki M   Aderem Alan A   Steinman Ralph M RM  

Journal of immunology (Baltimore, Md. : 1950) 20090201 3

Dendritic cells (DCs) are professional APCs that can control immune responses against self and altered self, typically foreign, determinants. DCs can be divided into several subsets, including CD8alpha(+) and CD8alpha(-) DCs. These subsets possess specific functions. For example, mouse splenic CD8alpha(+), but not CD8alpha(-) DCs selectively take up dying cells and cross-present cell-associated Ags to naive T cells. In this study, we identified genes that were more expressed in CD8alpha(+) than  ...[more]

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