Cardiac precursor differentiation from hESCs by lentiviral promoter drug selection

ABSTRACT: Several of the essential core transcriptional control elements in human embryonic stem cells (ESCs) have been identified, but the production and function of alternative isoforms in self-renewal, pluripotency and tissue lineage specification remain largely unknown. We have modified the H9 ESC line to allow for drug selection of human pluripotent ESCs and cardiac progenitors. Exon-level microarray expression data from undifferentiated ESCs and day 40 cardiac precursors were used to identify differentially expressed and alternative splice isoforms during differentiation. Keywords: comparison RNA from a homogenous population of undifferentiated hESCs (REX1-neo promoter drug selection) and differentiated day 40 cardiomyocytes (alpha MHC-puro promoter drug selection) was isolated and profiled with exon-tiling arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Nathan Salomonis 

PROVIDER: E-GEOD-13297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress