Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiling in cardiac AngII-overexpressing mice (TG1306/1R)

ABSTRACT: Agonist-induced cardiac hypertrophy in TG1306/1R transgenic mice with cardiac angiotensin II overproduction leads to a gradual transition from a compensatory hypertrophic state to heart failure. To gain insight into the molecular mechanisms that play a role in maintaining cardiac integrity in the diseased heart, we performed a comparative study of gene expression between wild-type (WT) and transgenic (TG) hearts using microarray analysis. TG1306/1R transgenics were separated into two phenotypic groups (hypertrophic and dilated) based on morphological parameters (cardiac weight index and histology) and either a moderate (hypertrophic) or a high (dilated) upregulation of molecular markers for hypertrophy and failure, and compared to age and sex-matched wild-types. In this series, twelve 60 week old male TG1306/1R mice were separated into 3 groups: WT1-4= Four Wild-types TG1306/1R littermates genetically negative for the transgene (-/-) Hyp1-4= Four TG1306/1R mice heterozygote for the transgene (-/+) and developing a concentric hypertrophic phenotype* Dil1-4 = Four TG1306/1R mice heterozygote for the transgene (-/+) and developing a dilated cardiac phenotype* This series contains 4 biological replicates for the following triangulation: WT is compared to Hyp, WT is compared to Dil, and Hyp is compared to Dil. * For further information read: Domenighetti AA, Wang Q, Egger M, Richards SM, Pedrazzini T, Delbridge LM. Angiotensin II-mediated phenotypic cardiomyocyte remodeling leads to age-dependent cardiac dysfunction and failure. Hypertension. 2005 Aug;46(2):426-32. [PMID: 15998712]

ORGANISM(S): Mus musculus

SUBMITTER: Andrea Domenighetti

PROVIDER: E-GEOD-13336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Control of the adaptive response of the heart to stress via the Notch1 receptor pathway.

Croquelois Adrien A Domenighetti Andrea A AA Nemir Mohamed M Lepore Mario M Rosenblatt-Velin Nathalie N Radtke Freddy F Pedrazzini Thierry T

The Journal of experimental medicine 20081208 13

In the damaged heart, cardiac adaptation relies primarily on cardiomyocyte hypertrophy. The recent discovery of cardiac stem cells in the postnatal heart, however, suggests that these cells could participate in the response to stress via their capacity to regenerate cardiac tissues. Using models of cardiac hypertrophy and failure, we demonstrate that components of the Notch pathway are up-regulated in the hypertrophic heart. The Notch pathway is an evolutionarily conserved cell-to-cell communica ...[more]

PMID: 19064701

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Dataset Information

Gene expression profiling in cardiac AngII-overexpressing mice (TG1306/1R)

Publications

Control of the adaptive response of the heart to stress via the Notch1 receptor pathway.

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