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Characterisation of modular response of E. coli Keio collection mutant library to acid adaptation

ABSTRACT: To study the dynamics of acid adaptation in E.coli Keio collection mutant library at pH7 and pH5.5 after 15 minutes of adaptation Keywords: Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and at pH5.5 after 15 minutes of adaptation

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Nil Turan 

PROVIDER: E-GEOD-13361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A systems biology approach sheds new light on Escherichia coli acid resistance.

Stincone Anna A   Daudi Nazish N   Rahman Ayesha S AS   Antczak Philipp P   Henderson Ian I   Cole Jeffrey J   Johnson Matthew D MD   Lund Peter P   Falciani Francesco F  

Nucleic acids research 20110619 17

In order to develop an infection, diarrhogenic Escherichia coli has to pass through the stomach, where the pH can be as low as 1. Mechanisms that enable E. coli to survive in low pH are thus potentially relevant for pathogenicity. Four acid response systems involved in reducing the concentration of intracellular protons have been identified so far. However, it is still unclear to what extent the regulation of other important cellular functions may be required for survival in acid conditions. Her  ...[more]

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