Project description:Enterohemorrhagic Escherichia coli (EHEC) is a gram negative enteric bacterial pathogen that can cause hemorrhagic colitis and heamolytic uremic syndrome (HUS) in humans and is the cause of bloody diarrhoea and acute renal failure in children. We have studied the transcriptional response of a colon cell line (CaCo2) to infection by EHEC and another closely related enteric pathogen Enteropathogenic Escherichia coli (EPEC) and compared its response to a cervical cell line (Hela). We carried out microarray analysis on CaCo2 infected with EHEC O157H:7 EDL933 and EPEC E2348/69 at 4 hours of infection and analysis on Hela infected with EHEC also at 4 hours of infection CaCo2 cells and Hela cells were grown to 80% confluency and infected with the bacteria for 4 hours before samples were collected for microarray analysis.
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 1hr every 5min.
Project description:Enterohemorrhagic Escherichia coli (EHEC) is a gram negative enteric bacterial pathogen that can cause hemorrhagic colitis and heamolytic uremic syndrome (HUS) in humans and is the cause of bloody diarrhoea and acute renal failure in children. We have studied the transcriptional response of a colon cell line (CaCo2) to infection by EHEC and compared its profile to infection by EHEC shocked in acid at pH 2.5. We carried out microarray analysis on CaCo2 infected with EHEC O157H:7 EDL933 and EHEC shocked at pH 2.5 at 4 hours post infection. CaCo2 cells were grown to 80% confluency and infected with acid shocked and unschocked bacteria for 4 hours before samples were collected for microarray analysis.
Project description:To study the dynamics of acid adaptation in E.coli Keio collection mutant library at pH7 and pH5.5 after 15 minutes of adaptation Keywords: Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and at pH5.5 after 15 minutes of adaptation
Project description:A microarray analysis was performed to compare the global gene expression profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells. SKOV-3 cells were treated with or without C-CPE for 72 hours, and total RNA was extracted and microarray was perfomed to compare the gene profiling changes between C-CPE treated- and untreated- cells. The experiment was performed in triplicate.
Project description:A microarray analysis was performed to compare the global gene expression profile between CLDN4-overexpressing (Control) and CLDN4-silencing SKOV-3 ovarian cancer cells. CLDN4 gene was knocked down by CLDN4 siRNA lentivirus. Total RNA was extracted and microarray was perfomed to compare the gene profiling changes between CLDN4-overexpressing (Control) and CLDN4-silencing cells. The experiment was performed in triplicate.
Project description:Clinical evidence has shown that electromagnetic fields produce a benefical therapeutic result for wounds, however little is still known about their exact mechanism of action. Moreover, clinical results concerning skin tissue restoration are still debated. In the present study, we carried out gene expression profiling of a human keratinocyte line (HaCaT) submitted to 1 hour of ELF-EMF (frequency 50Hz, intensity 1 mT) in order to identify up- and downregulated genes by this stimulus, and to verify the presence of specific molecular pathways activation. Most of the genes modulated were involved in mechanisms such as protein synthesis. In particular, these genes are related to Mammalian target of Rapamycin (mTOR), which has been identified as a kinase with a pivotal role in cellular proliferation and survival in mammals. In this study, we analyzed the expression profiles of 3 biological replicates of HaCaT cells exposed to ELF-EMF (frequency 50Hz, intensity 1 mT) for 1 hour. All HaCaT cells exposed to ELF-EMF RNAs were hybridized against control sham-exposed HaCaT cells RNAs. Each biological sample was repeated with a technical replicate (extraction-labeling) and a dye-swap experiment.