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Transcription profiling of Arabidopsis red 35S:PAP1 plants to investigate environmental regulation of leaf colour

ABSTRACT: Examine global gene expression patterns in control and 35S:PAP1 Arabidopsis plants upon environmental perturbation (light and temperature) over the course of the experiments. Experiment Overall Design: Red coloured 35S:PAP1, and empty vector control, plants of Arabidopsis thaliana Columbia were grown under room temperature, high light (RTHL, 22Â°C, 440 Âµmol m-2 s-1 irradiance) conditions to promote red leaf colouration. The growth conditions were then changed to a high day temperature, low light (HTLL: 30Â°C day, 150 Âµmol m-2 s-1 irradiance) stress treatment for six days, during which time the 35S:PAP1 leaves lost much of their red colouration, turning green. The growth conditions were then returned to a low temperature high light (LTHL, 15Â°C, 62% RH, 440 Âµmol m-2 s-1 irradiance) regime to restore the red colouration.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Mingshu Cao

PROVIDER: E-GEOD-13469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Environmental regulation of leaf colour in red 35S:PAP1 Arabidopsis thaliana.

Rowan Daryl D DD Cao Mingshu M Lin-Wang Kui K Cooney Janine M JM Jensen Dwayne J DJ Austin Paul T PT Hunt Martin B MB Norling Cara C Hellens Roger P RP Schaffer Robert J RJ Allan Andrew C AC

The New phytologist 20090112 1

* High-temperature, low-light (HTLL) treatment of 35S:PAP1 Arabidopsis thaliana over-expressing the PAP1 (Production of Anthocyanin Pigment 1) gene results in reversible reduction of red colouration, suggesting the action of additional anthocyanin regulators. High-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LCMS) and Affimetrix-based microarrays were used to measure changes in anthocyanin, flavonoids, and gene expression in response to HTLL. * HTLL treatmen ...[more]

PMID: 19192188

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Project description:The cold acclimation process is regulated by many factors like ambient temperature, day length, light intensity, or hormonal status. Experiments with plants grown under different light-quality conditions indicate that the plant response to cold is also a light-quality-dependent process. Here, the role of light quality in the cold response was studied in one-month-old Arabidopsis thaliana (Col‐0) plants exposed for one week to 4 °C at short‐day conditions under white (100 and 20 μmol m‐2s‐1), blue or red (20 μmol m‐2s‐1) light conditions. An upregulated expression of CBF1, an inhibition of photosynthesis, and an increase in membrane damage showed that blue light enhanced the effect of low temperature. Interestingly, cold-treated plants under blue and red light showed only limited freezing tolerance compared to white light cold-treated plants. Next, the specificity of the light quality signal in cold response was evaluated in Arabidopsis accessions originating from different and contrasting latitudes. In all but one Arabidopsis accessions, blue light increased the effect of cold on photosynthetic parameters and electrolyte leakage. This effect was not found for Ws-0, which lacks functional CRY2 protein, indicating its role in the cold response. Proteomics data confirmed significant differences between red and blue light treated plants at low temperature and showed that the cold response is highly accession specific. In general, blue light increased mainly the cold-stress related proteins and red light induced higher expression of chloroplast-related proteins, which correlated with higher photosynthetic parameters in red light cold-treated plants. Altogether, our data suggest that light modulates two distinct mechanisms during the cold treatment - red light driven cell function maintaining program and blue light activated specific cold response. The importance of mutual complementarity of these mechanisms was demonstrated by significantly higher freezing tolerance of cold-treated plants under white light.

Dataset Information

Transcription profiling of Arabidopsis red 35S:PAP1 plants to investigate environmental regulation of leaf colour

Publications

Environmental regulation of leaf colour in red 35S:PAP1 Arabidopsis thaliana.

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