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Unknown,Transcriptomics,Genomics,Proteomics

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Pearl millet seedlings infected with rust, or treated with MeJA or SA

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE13478: Pearl millet seedlings treated with methyl jasmonate (MeJA) GSE13479: Pearl millet seedlings infected with rust (Puccinia substriata) GSE13480: Pearl millet seedlings treated with salicylic acid (SA) Refer to individual Series

ORGANISM(S): Pennisetum glaucum

SUBMITTER: Bridget Crampton

PROVIDER: E-GEOD-13481 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum).

Crampton Bridget G BG Hein Ingo I Berger Dave K DK

Molecular plant pathology 20090301 2

Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet ge ...[more]

PMID: 19236576

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Pearl millet seedlings infected with rust (Puccinia substriata)

Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

2010-05-14 | E-GEOD-13479 | biostudies-arrayexpress

Pearl millet seedlings treated with methyl jasmonate (MeJA)

Project description:Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

2010-05-14 | E-GEOD-13478 | biostudies-arrayexpress

Pearl millet seedlings treated with salicylic acid (SA)

Project description:Transcriptional profiling of SA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

2010-05-14 | E-GEOD-13480 | biostudies-arrayexpress

A Spatiotemporal Transcriptome Revealed Stalk Development in Pearl Millet

Project description:Pearl millet is a major cereal crop that feeds more than 90 million people worldwide in arid and semi-arid regions. The stalk phenotypes of Poaceous grasses are critical for their productivity and stress tolerance, however, the molecular mechanisms governing stalk development in pearl millet remained to be deciphered. In this study, we spatiotemporally measured 19 transcriptomes for stalk internodes of four different early developmental stages. Data analysis of the transcriptomes defined 4 developmental zones on the stalks and identified 12 specific gene sets with specific expression patterns across the zones. Using weighted gene co-expression network analysis (WGCNA), we found that 2 co-expression modules together with candidate genes were involved in stalk elongation and thickening of pearl millet. Among the elongation-related candidate genes, we established by SELEX that a MYB-family transcription factor PMF7G02448 can bind to the promoters of three cell wall synthases genes (CesAs). In summary, these findings provide insights into stalk development and offer potential targets for future genetic improvement of pearl millet.

2024-09-15 | GSE268902 | GEO

Pearl millet seedlings infected with rust (Puccinia substriata)

Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response

2009-01-01 | GSE13479 | GEO

Pearl millet seedlings treated with salicylic acid (SA)

Project description:Transcriptional profiling of SA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response

2009-01-01 | GSE13480 | GEO

Pearl millet seedlings treated with methyl jasmonate (MeJA)

Project description:Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response

2009-01-01 | GSE13478 | GEO

Proteomic analysis on different tissues of Pteria penguin

Project description:The winged pearl oyster, Pteria penguin, is a widely distributed and economically important bivalve with unique and primitive byssus. The byssogensis of this species is largely unknown. Here we report the lable free quantification analysis on different tissues of P. penguin.Through the analysis, we found that the process of byssogenesis requires ATP to provide energy among the differential proteins; and seven (Tenascin-X, laminin, papilin, fibrillin-1, spondin, protease inhibitors and Mucins) of the 31 proteins identified in the byssus were derived from ECM. Functions of extracellular matrix proteins can be correlated with the formation and the nature of the byssus.

2023-12-20 | PXD031701 | Pride

Comparison of the root tip transcriptome of 2 pearl millet lines with contrasted root soil aggregation

Project description:We analyzed global gene expression in the crown tip of 2 pearl millet (Pennisetum glaucum) inbred lines with high (line 249) and low (line 220) root soil aggregation using RNAseq. The obtective was to identify genes potentially associated with changes in rhizosheath formation.

2021-10-09 | GSE185425 | GEO

repaired shells of Pinctada fucata LC-MSMS

Project description:Shells of pearl oyster are natural biominerals with remarkable properties and can be repaired after damage, which are regulated by biomacromolcules especially shell matrix proteins (SMPs). Identifying SMPs is critical for further understanding the process. Although proteomic methods have been used to reveal the complex protein mixture in mature shells, the proteomics of repaired shells after shell damage have not been reported before. Here, we studied the SMPs of the repaired shells (prismatic layers) 5-10 days after shell damage in Pinctada fucata by integrating transcriptomics and proteomics and compared the microstructure difference between repaired and mature shells. Although the repaired shells are calcite, similar to mature shells, the microstructure of repaired shells has holes in the center of prisms with irregular and curved edges. In total, we found 49 SMPs from the repaired shells including some proteins only existing in mature nacreous layers. Peroxidase-like protein and beta-N-acetylhexosaminidase may be important players in repaired shells. In addition, they have the capability to affect CaCO3 crystallization process in vitro, altering the packing and reducing the crystallinity of crystals. This study could improve our understanding of shell repair process and lay the foundation for studying SMPs-controlled biomineralization.

2019-03-26 | PXD010130 | Pride

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