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Pearl millet seedlings treated with methyl jasmonate (MeJA)

ABSTRACT: Transcriptional profiling of MeJA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

ORGANISM(S): Pennisetum glaucum

SUBMITTER: Ingo Hein

PROVIDER: E-GEOD-13478 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum).

Crampton Bridget G BG Hein Ingo I Berger Dave K DK

Molecular plant pathology 20090301 2

Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet ge ...[more]

PMID: 19236576

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Pearl millet seedlings treated with salicylic acid (SA)

Project description:Transcriptional profiling of SA-treated pearl millet seedlings over time [0, 12, 24 and 48 hours post treatment (hpt)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

2010-05-14 | E-GEOD-13480 | biostudies-arrayexpress

Pearl millet seedlings infected with rust (Puccinia substriata)

Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response Loop design. All time points compared with time = 0 h in data analysis. Two biological replicates per sample, and one technical dye swap replicate.

2010-05-14 | E-GEOD-13479 | biostudies-arrayexpress

Pearl millet seedlings infected with rust, or treated with MeJA or SA

Project description:This SuperSeries is composed of the following subset Series: GSE13478: Pearl millet seedlings treated with methyl jasmonate (MeJA) GSE13479: Pearl millet seedlings infected with rust (Puccinia substriata) GSE13480: Pearl millet seedlings treated with salicylic acid (SA) Refer to individual Series

2010-05-14 | E-GEOD-13481 | biostudies-arrayexpress

Arabidopsis Col-5 challenged with Ralstonia solanacearum BCCF401

Project description:Arabidopsis plants were challenged with Ralstonia solanacearum isolate BCCF401 and expression profiles investigated during early and late wilt symptom development. Keywords: Disease state analysis A direct comparison was performed. Two replicate biological experiments were performed and two technical replicates with dye swap were included in each biological replicate.

2010-11-22 | E-GEOD-19178 | biostudies-arrayexpress

Analysis of gene expression in Arabidopsis thaliana after priming with BABA and infection with Pseudomonas syringae pv tomato DC3000

Project description:Arabidopsis thaliana (Col-0) plants were treated with BABA and gene expression differences to control plants were monitored after dip-inoculation with Pseudomonas syringae pv tomato DC3000. Keywords: transcript profiling, response to BABA-induced priming and infection 3 independant replicates were analyzed by two color co-hybridizations. Leaf RNA from Pseudomonas infected control plants (Cy3 labeled cDNA) was cohybridized with leaf RNA from Pseudomonas infected BABA pretreated plants (Cy5 labeled cDNA). Samples were collected 22 hours after bacterial inoculation. BABA pretreatment was performed two days before bacterial inoculation. To assess the effect of BABA alone on gene expression, leaf RNA from BABA treated plants (Cy5 labeled cDNA) was cohybridized with leaf RNA (Cy3 labeled cDNA) from water treated plants.

2010-11-19 | E-GEOD-16434 | biostudies-arrayexpress

Porphyromonas gingivalis W50 Heme-excess vs Heme-limitation

Project description:Transcription profiling of P. gingivalis W50 grown in continuous culture under conditions of heme-excess and heme-limitation. Reference design (using Cy5 labelled genomic DNA as the reference) to compare two conditions: heme-excess vs heme-limitation. Three samples for each condition, independently grown.

2010-05-16 | E-GEOD-13375 | biostudies-arrayexpress

Identification of Candida albicans genes differentially expressed in the presence of arachidonic acid

Project description:Although Candida species, more specifically C. albicans, are the most common pathogenic yeasts, little is known about the mechanism involved in eicosanoid production, a virulence factor in these organisms. This is an important area of research which may aid in the understanding of the complex interactions between host and pathogen and may possibly lead to the identification of novel antifungals or drug targets. In this study, genomic hybridization studies using C. albicans DNA microarrays were used to evaluate the regulation of C. albicans biofilm genes during incubation in the presence of arachidonic acid, the major precursor for prostaglandin E2 production. The results obtained indicated that the genes differentially expressed had diverse functions not normally required for cell growth. Two-condition experiment, Control biofilms vs. Arachidonic acid treated biofilms at different time intervals. Biological replicates: 3 Control, 3 Arachidonic acid treated, independently grown and harvested. Technical replicate: dye swap included. Triplicate per array.

2012-03-14 | E-GEOD-36436 | biostudies-arrayexpress

Transcription profiling of Treponema denticola biofilms vs cells grown planktonically

Project description:Comparison of T. denticola cells grown as a biofilm to cells grown planktonically

2009-12-01 | E-MEXP-2266 | biostudies-arrayexpress

Expression profiling of cotton (Gossypium hirsutum L. TM1) fiber transcriptome during the development

Project description:In order to study gene expression at the genomic level during elongation and secondary cell wall synthesis of upland cotton fiber, oligonucleotide microarrays were employed. RNA was isolated from fibers in 7 different time points beginning prior to peak fiber expansion, continuing through termination of fiber expansion and ending at peak cellulose synthesis (5, 8, 10, 14, 17, 21, and 24dpa). The arrays contained ~25,000 oligonucleotides representing ~12,200 genes designed from a fiber EST database during peak cell expansion. Dynamic changes in gene expression were analyzed in a developmental context to identify stage-specific biological processes and pathways likely to be crucial to cell polar elongation or cellulose biosynthesis and secondary cell wall biogenesis. Genes with significant changes in expression relative to any preceding time point were identified (moderated t-statistics, adjusted p-value <0.05) for each developmental time point with an expected false discovery rate for multiple testing <5% A bi-directional double-loop experimental design was adopted for the microarray analysis (Kerr and Churchill, 2001; Glonek, 2004) to analyze all possible significant changes in gene expression between any two developmental time points,. The double loop design guarantees that the two time points before and after each individual time point will have direct comparisons with multiple paths available to compare any two points. Self-hybridization control experiments between independent RNA isolations for each developmental stage demonstrated a high degree of reproducibility

2010-05-26 | E-GEOD-11693 | biostudies-arrayexpress

Analysis of the transcriptional response of Candida parapsilosis following exposure to farnesol

Project description:We used the results of a genome sequence survey to design and manufacture partial genomic microarrays that were applied to determining the transcriptional response of C. parapsilosis to the presence of exogenous farnesol. Keywords: drug response, farnesol, Transcriptional profiling was performed following exposure to 50 microM of farnesol compare to the control experiment (no treatment). Six independent experiment comparisons (including 3 dye swaps) were performed.

2010-06-11 | E-GEOD-6318 | biostudies-arrayexpress

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