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Divergent transcription from active promoters

ABSTRACT: Here we describe a novel class of short RNAs that indicate widespread divergent transcription at mammalian promoters Small RNA cloning and deep sequencing from human embryonic stem cells, derived neural progenitors and neurons

ORGANISM(S): Homo sapiens

SUBMITTER: Gene Yeo

PROVIDER: E-GEOD-13483 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Divergent transcription from active promoters.

Seila Amy C AC Calabrese J Mauro JM Levine Stuart S SS Yeo Gene W GW Rahl Peter B PB Flynn Ryan A RA Young Richard A RA Sharp Phillip A PA

Science (New York, N.Y.) 20081204 5909

Transcription initiation by RNA polymerase II (RNAPII) is thought to occur unidirectionally from most genes. Here, we present evidence of widespread divergent transcription at protein-encoding gene promoters. Transcription start site-associated RNAs (TSSa-RNAs) nonrandomly flank active promoters, with peaks of antisense and sense short RNAs at 250 nucleotides upstream and 50 nucleotides downstream of TSSs, respectively. Northern analysis shows that TSSa-RNAs are subsets of an RNA population 20 t ...[more]

PMID: 19056940

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Divergent transcription from active promoters

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Project description:This SuperSeries is composed of the SubSeries listed below.

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