Gene expression during early sporulation in Phytophthora infestans
Ontology highlight
ABSTRACT: Transcriptional changes during asexual sporangia formation by the late blight pathogen Phytophthora infestans were identified using microarrays representing 15,650 genes and RNA from sporulation time-courses, purified spores, and sporulation-defective strains. Results were confirmed by reverse transcription-polymerase chain reaction analyses of sporulation on artificial media and infected tomato. During sporulation, about 12% of genes were found to be up-regulated and 5% down-regulated. The most prevalent induced genes had functions in signal transduction, flagella assembly, cellular organization, metabolism, and molecular or vesicular transport. Distinct patterns of expression were discerned based on the kinetics of mRNA induction and their persistence in sporangia. For example, most flagella-associated transcripts were induced very early in sporulation and maintained in sporangia, while many participants in metabolism or small molecule transport were also induced early but had low levels in sporangia. Data from this study are a resource for understanding sporogenesis, which is critical to the pathogenic success of P. infestans and other oomycetes. Two arrays were analyzed for each type of biological material. For each of the replicates, RNA was extracted from 2-3 different cultures and pooled. Therefore, a total of 4-6 biological replicates were analyzed for each type of tissue.
ORGANISM(S): Phytophthora infestans
SUBMITTER: Howard Judelson
PROVIDER: E-GEOD-13580 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA