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Transcription profiling of mouse lung from T1 alpha (-/-) vs. wild type at fetal and term developmental stages

ABSTRACT: We compared gene expression in T1alpha (-/-) lungs vs wild type at dpc 18.5 when the phenotype is moderate and at term when the phenotype is more severe. Lungs present narrow and irregular alveolar spaces, thicker mesenchyme, reduced number of attenuated type I cells, normal numbers of type II cells and secreted surfactant. At term, but not a dpc 18.5, there is increased proliferation of distal cells. We compared gene expression of T1alpha null mutant lungs to wild type lungs at dpc 18.5. The mutant phenotype at this developmental stage is moderate but the alveolar sacs are narrower than normal.

ORGANISM(S): Mus musculus

SUBMITTER: Maria Isabel Ramirez

PROVIDER: E-GEOD-1363 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Alterations in gene expression in T1 alpha null lung: a model of deficient alveolar sac development.

Millien Guetchyn G Spira Avrum A Hinds Anne A Wang Junling J Williams Mary C MC Ramirez Maria I MI

BMC developmental biology 20060725

<h4>Background</h4>Development of lung alveolar sacs of normal structure and size at late gestation is necessary for the gas exchange process that sustains respiration at birth. Mice lacking the lung differentiation gene T1alpha [T1alpha(-/-)] fail to form expanded alveolar sacs, resulting in respiratory failure at birth. Since little is known about the molecular pathways driving alveolar sacculation, we used expression microarrays to identify genes altered in the abnormal lungs and, by inferenc ...[more]

PMID: 16869965

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Gene expression in T1alpha null mutant lungs compared to wild type at dpc 18.5 and term

Project description:We compared gene expression in T1alpha (-/-) lungs vs wild type at dpc 18.5 when the phenotype is moderate and at term when the phenotype is more severe. Lungs present narrow and irregular alveolar spaces, thicker mesenchyme, reduced number of attenuated type I cells, normal numbers of type II cells and secreted surfactant. At term, but not a dpc 18.5, there is increased proliferation of distal cells. We compared gene expression of T1alpha null mutant lungs to wild type lungs at dpc 18.5. The mutant phenotype at this developmental stage is moderate but the alveolar sacs are narrower than normal. Keywords: other

2004-09-15 | GSE1363 | GEO

Mus musculus

Project description:Gene expression in T1alpha null mutant lungs compared to wild type at dpc 18.5 and term

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Transcriptomic analyses of fetal (18.5 dpc) and adult (3 months) mouse testes after in utero BPA and/or ionizing radiations exposure(s)

Project description:Despite the fact that we are constantly exposed to various environmental compounds, very few studies explore the impact of combined exposure to physical and chemical pollutants on reproductive health. Until now, assessment of pollutants is mostly based on the evaluation of single pollutant or combination of chemicals with common features and modes of action. In this context, numerous studies have demonstrated that steroidogenesis and gametogenesis, the main testicular functions, are well-known to be sensitize by endocrine disruptors (as Bisphenol A) and DNA-damaging agents (as γ-rays) respectively. In this study, we aim to investigate short and long term testicular transcriptionnal alterations of combined fetal exposure to well-known environmental toxicants: γ-rays (RAD) and BPA. To discriminate specific signatures of BPA or RAD exposure after combined exposure and evidence the type of synergisms between this pollutants, we performed transcriptomic analyses on testis exposed to BPA or RAD alone and co-exposed with BPA and RAD and compared gene expression with control condition. For this, we exposed pregnant mice from 10.5 dpc to 18.5 dpc to 10µM of BPA (in drinking water) and/or we irradiated mice at 12.5 dpc to 0.2 Gy. We performed transcriptomic analyses on fetal testis (18.5 dpc) and adult testis (3 months) to evaluate short and long term cell response after in utero exposure.

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Expression data from E18.5 Igf1 -/- (homozygous mutant) and Igf1+/+ (normal wild type control) mouse lungs

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Low paternal dietary folate alters the sperm epigenome and is associated with negative pregnancy outcomes

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ROSA26Foxf1; Tie2-cre lung gene expression analysis.

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ROSA26Foxf1; Tie2-cre lung gene expression analysis.

Project description:Analysis of genes deregulated due to heterozygous overexpression of Foxf1 in endothelial and hematopoietic cells using Tie2-cre in embryonic day 18.5 mouse lungs. Total RNA obtained from three ROSA26Foxf1; Tie2-cre mouse lungs compared to three control ROSA26-lox-stop-lox (LSL) -Foxf1 mouse lungs.

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Transcriptome in alveolar epithelial progenitor cells isolated from adult human lung

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