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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wheat response to infection by Fusarium pseudograminearum

ABSTRACT: Crown rot of wheat, caused by Fusarium pseudograminearum and other Fusarium species is an important disease globally. To understand the host response to challenge by Fp, we examined gene exression changes in the stem base of the wheat variety Kennedy, following inoculation with macroconidia using the Affymetrix GeneChip Wheat Genome Array. Induced genes included mainly those with defensive functions such as genes encoding anti-microbial proteins as well as oxidative stress-related proteins, signalling molecules, and proteins involved in both primary and secondary metabolism. This study is the first comprehensive analysis of the wheat transcriptome during crown rot infection and provides new insights into the host processes involved in plant defence against this pathogen. Experiment Overall Design: There are six samples, three F. pseudograminearum inoculated samples and three mock inoculated samples. Each sample consists of 2cm of stem base from approximately 20 plants.

ORGANISM(S): Triticum aestivum

SUBMITTER: Brendan Kidd

PROVIDER: E-GEOD-13660 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis of the wheat response to infection by Fusarium pseudograminearum

Project description:Crown rot of wheat, caused by Fusarium pseudograminearum and other Fusarium species is an important disease globally. To understand the host response to challenge by Fp, we examined gene exression changes in the stem base of the wheat variety Kennedy, following inoculation with macroconidia using the Affymetrix GeneChip Wheat Genome Array. Induced genes included mainly those with defensive functions such as genes encoding anti-microbial proteins as well as oxidative stress-related proteins, signalling molecules, and proteins involved in both primary and secondary metabolism. This study is the first comprehensive analysis of the wheat transcriptome during crown rot infection and provides new insights into the host processes involved in plant defence against this pathogen.

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Differential transcriptome analysis of two wheat genotypes in their response to Fusarium head blight

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Transcription analysis by RNAseq of adult wheat after inoculation with Puccinia graminis f. sp. tritici

Project description:The RNA sequencing analysis was undertaken to investigate the transcriptomic changes in adult wheat inoculated with Puccinia graminis f. sp. tritici the causal agent of stem rust disease. The project firstly aims to compare gene expression in one susceptible wheat line with two wheat lines exhibiting adult plant resistance to the stem rust. Secondly, the project aims to examine the temporal changes in gene expression in wheat after inoculation. Wheat plants was grown until maturity under greenhouse conditions. Plants were inoculated with Puccinia graminis f. sp. tritici and the flag leaf sheath sampled for RNA sequencing. The project aims to give essential insight into the adult plant resistance response in wheat to Puccinia graminis f. sp. tritici inoculation.

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Triticum aestivum Transcriptome or Gene expression

Project description:Identify genes underlying the Fusarium crown rot resistance locus and find out if expressed genes associated with resistance to Fusarium crown rot were related to those observed by others for Fusarium head blight.

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Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affecting the flowering heads (or spikes). A FHB resistance locus has been identified on the chromosome 7E of the wild wheat relative Thinopyrum elongatum (Th.e.). That chromosome (7E) or a long arm fragment of it (7EL) have been transferred as additions in the wheat background 'Chinese Spring' (CS). The two addition lines are resistant to FHB while 'Chinese Spring' is moderately susceptible to it. The mechanism of resistance is not known. The analysis of this work is published in the Canadian Journal of Plant Pathology (Wang et al, 2010). We used the wheat microarray to determine the global expression profil in inoculated spikelets of the addition and parental lines, after water or Fg treatment, with samplings at 2 and 4 days after inoculation (DAI).

2010-06-23 | E-GEOD-21386 | biostudies-arrayexpress

Proteomic analysis of the apoplast reveals a differential cold acclimation response among winter wheat crown tissues

Project description:The crown is the critical region for survival of winter wheat exposed to low temperature stresses. When wheat is exposed to non-freezing low temperatures, they can increase their freezing tolerance (cold acclimation, ACC). Changes within the apoplast are thought to be crucial for acquisition of freezing tolerance. However, how individual tissues within the ccrown, namely the shoot apical meristem (SAM, responsible for new shoot growth) and vascular transition zone (VTZ, located at the base of the crown)enhance tolerance to freezing has not yet been characterized. In the present study, we conducted shotgun proteomic analysis of the apoplast fluid to investigate ACC-induced proteins in the SAM and VTZ.

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Global gene expression analysis of FHB disease spreading mechanism in wheat

Project description:The Fusarium graminearum is responsible for Fusarium head blight (FHB) disease in wheat which makes unsuitable for end-use quality of wheat. For that reason several studies have been aiming to develop FHB resistant cultivars. In order to clarify possible wheat response mechanism, global gene expression analysis in response to the pathogen was undertaken. The study was conducted using three genotype of wheat, Japanese cultivar Nobeokabouzu-komugi (Highly resistant), cv. Sumai 3 (Resistant) and cv. Gamenya (Susceptible) which florets were inoculated Fusarium graminearum M-bM-^@M-^\H-3M-bM-^@M-^] strain during the flowering stage. For microarray analysis used samples of 3 and 7 days after inoculation (DAI) and Agilent wheat custom array 4x38k. Over 1400 genes were differentially up-regulated in Nobeokabouzu-komugi at 3 DAI followed by Sumai 3 and minimum expression in Gamenya, whereas at 7 DAI in Sumai 3 observed high expression and minimum in Gamenya. Further narrowing down by removing commonly expressed genes in three genotypes and grouping according to the gene function, identified differentially high expression of genes involved in detoxification process such as multidrug resistant rpoteins, UDP-glycosyltransferase and ABC transporters in Nobeokabouzu-komugi at 3 DAI. However in Sumai 3 found many localized defense-related genes such as peroxidase, proteases and genes involved in plant cell wall defense at 7 DAI. Following in silico analysis, revealed those genes were located in different chromosomes. This finding showed the differentiation of molecular response mechanism among the FHB resistant cultivars in response to the pathogen. F. graminearum inoculated (3 and 7 days after inoculation) and water inoculated Mock samples were used for cRNA synthesis and further hybridization with 3 biological replications. Total 12 samples per genotype

2015-01-30 | E-GEOD-59721 | biostudies-arrayexpress

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