Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiling after RNA interference of CXCR4 in human ovarian cancer cell line IGROV-1.

ABSTRACT: In the past three years the role of inflammatory cytokines and chemokines in tumour promotion and progression has been intensively studied. The chemokine receptor CXCR4 and its ligand CXCL12 are commonly expressed in malignant cells from primary tumours, metastases and also in malignant cell lines. To investigate the biological significance of this receptor/ligand pair, we knocked-down CXCR4 expression in ovarian cancer cell line IGROV-1 using shRNA, and established stable cell lines. Using Affymetrix microarrays we compared in vitro gene expression in parental IGROV-1 and IGROV-Mock cells with two clones of IGROV-shCXCR4 cells. Gene Set Enrichment Analysis (GSEA) of those genes which were altered by RNA interference of CXCR4 revealed evidence for a cell autonomous signaling network involving CXCR4, TNF-a, IL6 and Notch pathways in ovarian cancer cells. Experiment Overall Design: The Affymetrix GeneChip Human Genome U133Plus 2.0 arrays were used to define gene expression profiles in each cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Hagen Kulbe

PROVIDER: E-GEOD-13763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.

Kulbe Hagen H Chakravarty Probir P Leinster D Andrew DA Charles Kellie A KA Kwong Joseph J Thompson Richard G RG Coward Jermaine I JI Schioppa Tiziana T Robinson Stephen C SC Gallagher William M WM Galletta Laura L Salako Michael A MA Smyth John F JF Hagemann Thorsten T Brennan Donal J DJ Bowtell David D DD Balkwill Frances R FR

Cancer research 20111107 1

Constitutive production of inflammatory cytokines is a characteristic of many human malignant cell lines; however, the in vitro and in vivo interdependence of these cytokines, and their significance to the human cancer microenvironment, are both poorly understood. Here, we describe for the first time how three key cytokine/chemokine mediators of cancer-related inflammation, TNF, CXCL12, and interleukin 6, are involved in an autocrine cytokine network, the "TNF network," in human ovarian cancer. ...[more]

PMID: 22065722

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Dataset Information

Gene expression profiling after RNA interference of CXCR4 in human ovarian cancer cell line IGROV-1.

Publications

A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.

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