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Expression profiling of Ovine Herpesvirus 2-infected lymph nodes from Bos taurus

ABSTRACT: We hypothesized that the gene expression pattern of OvHV-2 may be important to understand the pathogenesis of malignant catarrhal fever (MCF). Therefore, RNA was extracted from lymph nodes of animals with MCF and healthy controls to be analyzed by a custom-made microarray. Two regions on the viral genome were transcriptionally active, one encoding a homologue to the latency-associated nuclear antigen (ORF73) of other gamma herpesviruses, the other with no predicted open reading frame. Keywords: Disease state analysis Two biological replicates with two technical replicates each, with one of the technical replicates being a dye-swap.

ORGANISM(S): Bos taurus

SUBMITTER: Claudia Meier-Trummer

PROVIDER: E-GEOD-13853 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Malignant catarrhal fever of cattle is associated with low abundance of IL-2 transcript and a predominantly latent profile of ovine herpesvirus 2 gene expression.

Meier-Trummer Claudia S CS Rehrauer Hubert H Franchini Marco M Patrignani Andrea A Wagner Ulrich U Ackermann Mathias M

PloS one 20090715 7

<h4>Background</h4>Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease through immunopathogenic pathways. Lymphocytes are the main target of the virus. However, the pathogenic basis of the disease is still mysterious.<h4>Methods/findings</h4>We hypothesized that the gen ...[more]

PMID: 19603070

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