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Transcription profiling of mouse gastro intestinal tract after conventional and germ free treatments

ABSTRACT: Whole tissues corresponding to the corpus, jejunum, descending colon and rectum were dissected from each mouse, washed with ice-cold PBS, placed into 2ml of RNA-later and flash-frozen in liquid nitrogen. The corpus was defined as the part of the stomach different from the antrum (distinguished by a difference in colour). The jejunum was defined as the tissue between the first 4 cm and the last 2 cm of the small intestinal tube. The descending colon and the rectum were defined as the last 3 cm of the digestive tube, where the first 2 cm was the descending colon and the last centimetre was the rectum.

ORGANISM(S): Mus musculus

SUBMITTER: James Holzwarth

PROVIDER: E-GEOD-1392 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Impact of commensal microbiota on murine gastrointestinal tract gene ontologies.

Mutch David M DM Simmering Rainer R Donnicola Dominique D Fotopoulos Grigorios G Holzwarth James A JA Williamson Gary G Corthésy-Theulaz Irène I

Physiological genomics 20040629 1

The gastrointestinal tract (GIT) of eukaryotes is colonized by a vast number of bacteria, where the commensal microbiota play an important role in defining the healthy gut. To investigate the influence of commensal bacteria on multiple regions of the host GIT transcriptome, the gene expression profiles of the corpus, jejunum, descending colon, and rectum of conventional (n = 3) and germ-free mice (n = 3) were examined using the Affymetrix Mu74Av2 GeneChip. Differentially regulated genes were ide ...[more]

PMID: 15226484

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