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Effects of labelling fetal MSC with MRI Particles: MGIO and Ferucarbotran

ABSTRACT: Cell tracking is enabled by incubating ex vivo cells with commercially/clinically available MRI particulate label, such as ferucarbotran. However, the uptake by non-phagocytic cells, such as mesenchymal stem cell (MSC) is poor, and the detection by MRI is impeded. MGIO is a new label that is efficiently taken up by MSC. The proliferation and differentiation capacity of labelled cells are usually assessed to determine cytotoxicity. In this study, we compared the global gene expression profiles of mock-labelled, ferucarbotran-labelled and MGIO-labelled fetal MSC. Experiment Overall Design: Fetal MSC were labelled at passage 5, washed with PBS to remove excess labels, detached by trypsin, pelleted by centrfugation and lysed for RNA extraction with the RNeasy mini kit (Qiagen) in triplicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Eddy SM Lee 

PROVIDER: E-GEOD-13975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microgel iron oxide nanoparticles for tracking human fetal mesenchymal stem cells through magnetic resonance imaging.

Lee Eddy S M ES   Chan Jerry J   Shuter Borys B   Tan Lay Geok LG   Chong Mark S K MS   Ramachandra Durrgah L DL   Dawe Gavin S GS   Ding Jun J   Teoh Swee Hin SH   Beuf Olivier O   Briguet Andre A   Tam Kam Chiu KC   Choolani Mahesh M   Wang Shih-Chang SC  

Stem cells (Dayton, Ohio) 20090801 8

Stem cell transplantation for regenerative medicine has made significant progress in various injury models, with the development of modalities to track stem cell fate and migration post-transplantation being currently pursued rigorously. Magnetic resonance imaging (MRI) allows serial high-resolution in vivo detection of transplanted stem cells labeled with iron oxide particles, but has been hampered by low labeling efficiencies. Here, we describe the use of microgel iron oxide (MGIO) particles o  ...[more]

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