Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Effect of L1CAM Knockout on Gene Expression in the Cerebellum

ABSTRACT: Cerebellum from post-natal day 11 L1 knockout mice on the 129Sv background were compared to wild type littermates. The original goal of the study was to determine if there was compensation from other L1 family members or alterations in cell survival or apoptosis. Interestingly no major changes were detected in those families or pathways. The microarray experiment was conducted by extracting total RNA from the cerebellums of three postnatal day 11 congenic 129Sv L1 knock outs and three of their wild type siblings utilizing Qiagen's RNeasy Mini kit (# 74104). Therefore, each genotype, L1KO and wildtype, had three replicates. Samples were processed at the Case Western Reserve University Cancer Center Gene Expression Array Core Facility and hybridized to Affymetrix Murine Genome U74AV2 gene chips.

ORGANISM(S): Mus musculus

SUBMITTER: Vance Lemmon

PROVIDER: E-GEOD-13984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A modifier locus on chromosome 5 contributes to L1 cell adhesion molecule X-linked hydrocephalus in mice.

Tapanes-Castillo Alexis A Weaver Eli J EJ Smith Robin P RP Kamei Yoshimasa Y Caspary Tamara T Hamilton-Nelson Kara L KL Slifer Susan H SH Martin Eden R ER Bixby John L JL Lemmon Vance P VP

Neurogenetics 20090630 1

Humans with L1 cell adhesion molecule (L1CAM) mutations exhibit X-linked hydrocephalus, as well as other severe neurological disorders. L1-6D mutant mice, which are homozygous for a deletion that removes the sixth immunoglobulin-like domain of L1cam, seldom display hydrocephalus on the 129/Sv background. However, the same L1-6D mutation produces severe hydrocephalus on the C57BL/6J background. To begin to understand how L1cam deficiencies result in hydrocephalus and to identify modifier loci tha ...[more]

PMID: 19565280

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Project description:Mastitis is a multietiological complex disease, which is defined as inflammation of parenchyma of mammary glands. Bacterial infection is the predominant cause of mastitis, though fungal, viral and mycoplasma infections also have been reported. Based on severity of the disease, mastitis can be classified into- subclinical, clinical and chronic forms. Bacterial pathogens from the fresh cow milk were isolated and classified by standard microbiological tests and multiplex PCR. Epidemiological studies have shown that Escherichia coli is the 2nd largest mastitic pathogen after Staphylococcus aureus in India. Based on Enterobacterial Repetitive Intergenic Consensus (ERIC) -PCR profile and presence of virulence genes, a field isolate of E. coli was used for intramammary inoculation in lactating mice. Histopathological examination of H&E stained sections showed severe infiltration of polymorphonuclear neutrophils (PMNs), mononuclear inflammatory cells in the alveolar lumen and also in interstitial space and necrosis of alveolar epithelial cells after 24h. Western blot and immunohistochemical analysis of the mice mammary tissues have shown significant hyperacetylation at histone H3Lys14 residue of both mammary epithelial cells and migrated inflammatory cells. Quantitative real-time PCR and genome-wide gene expression profile in the E. coli infected mice mammary tissue revealed differential expression of genes related to inflammation, immunity, antimicrobial peptide expression, acute phase response and oxidative stress response. Expression levels of milk proteins were also suppressed. ChIP assay from the paraffinized tissues have shown selective enrichment of acetylated histone H3K14 and H4K8 at the promoters of over expressed genes. These data suggests that E. coli infection in the mice mammary tissue leads to histone hyperacetylation at the immune gene promoters which is pre-requisite for expression of inflammatory genes to mount drastic immune response. Abdominal mammary glands of 7 Day post partum lactating female Swiss albino mice was challenged with a pure culture of E. coli isolated from cow milk. Mammary tissues were harvested after 24h of infection. Total RNA was isolated by Qiagen RNeasy mini columns and used for global gene expression and RT-PCR analysis. For RT-PCR, cDNAs corresponding to total mRNAs were synthesized using Oligo-dT primer. Global gene expression analysis were done by single colour labelling of total RNA and hybridization to Agilent 8x60k mouse Microarray.

Dataset Information

Effect of L1CAM Knockout on Gene Expression in the Cerebellum

Publications

A modifier locus on chromosome 5 contributes to L1 cell adhesion molecule X-linked hydrocephalus in mice.

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