Unknown,Transcriptomics,Genomics,Proteomics

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SiRNA profiling of human steroid-deprived LNCaP cells were transfected with control or ART-27 siRNA to investigate the genome-wide impact of ART-27 loss on androgen-regulated transcription


ABSTRACT: The androgen receptor (AR) directs diverse biological processes through interaction with coregulators such as androgen receptor trapped clone-27 (ART-27). The impact of ART-27 on genome-wide transcription was examined. The studies indicate that loss of ART-27 enhances expression of many androgen-regulated genes, suggesting that ART-27 inhibits gene expression. Surprisingly, classes of genes that are upregulated upon ART-27 depletion include regulators of DNA damage checkpoint and cell cycle progression, suggesting that ART-27 functions to keep expression levels of these genes low. Experiment Overall Design: Steroid-deprived LNCaP cells were transfected with control or ART-27 siRNA and stimulated with ethanol vehicle or 10 nM R1881 for 18 hrs. 8 samples, 4 conditions, 2 replicates per condition.

ORGANISM(S): Homo sapiens

SUBMITTER: Susan Logan 

PROVIDER: E-GEOD-14043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide impact of androgen receptor trapped clone-27 loss on androgen-regulated transcription in prostate cancer cells.

Nwachukwu Jerome C JC   Mita Paolo P   Ruoff Rachel R   Ha Susan S   Wang Qianben Q   Huang S Joseph SJ   Taneja Samir S SS   Brown Myles M   Gerald William L WL   Garabedian Michael J MJ   Logan Susan K SK  

Cancer research 20090324 7


The androgen receptor (AR) directs diverse biological processes through interaction with coregulators such as AR trapped clone-27 (ART-27). Our results show that ART-27 is recruited to AR-binding sites by chromatin immunoprecipitation analysis. In addition, the effect of ART-27 on genome-wide transcription was examined. The studies indicate that loss of ART-27 enhances expression of many androgen-regulated genes, suggesting that ART-27 inhibits gene expression. Surprisingly, classes of genes tha  ...[more]

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