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Transcription profiling of human colorectal cancer cell lines transfected with homeobox gene CDX1 cDNA to identfiy downstream targets

ABSTRACT: This study is to identify downstream targets of homeobox gene CDX1. The study assayed the expression of 2 pairs of stably transfected colorectal cancer cell lines: The CDX1 nonexpressing CRC cell line HCT116 was stably transfected with either CDX1 cDNA in the pRC/CMV expression vector (HCT116-CDX1) or with vector control (HCT116-Vec). The CDX1-expressing CRC cell line LS174T was similarly transfected with either a pSilencer vector containing a short sequence of CDX1 siRNA (LS174T-siRNA) , or a pSilencer vector containing a scrambled siRNA sequence as a control (LS174T-Vec). Experiment Overall Design: 2 pairs of colorectal cancer cell lines were used for comparison

ORGANISM(S): Homo sapiens

SUBMITTER: Carol Chan

PROVIDER: E-GEOD-14380 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1

Project description:This study is to identify downstream targets of homeobox gene CDX1. The study assayed the expression of 2 pairs of stably transfected colorectal cancer cell lines: The CDX1 nonexpressing CRC cell line HCT116 was stably transfected with either CDX1 cDNA in the pRC/CMV expression vector (HCT116-CDX1) or with vector control (HCT116-Vec). The CDX1-expressing CRC cell line LS174T was similarly transfected with either a pSilencer vector containing a short sequence of CDX1 siRNA (LS174T-siRNA) , or a pSilencer vector containing a scrambled siRNA sequence as a control (LS174T-Vec).

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Project description:HCT116 (CRC): control siRNA vs. ZNF746 siRNA Transfected

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Expression data for human colon cancer cell line HCT116 transfected with siRNA targeting YAP1.

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HCT116 (CRC): control siRNA vs. ZNF746 siRNA Transfected

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RNA-seq of FOXQ1-transfected HCT116 cells to understand the Wnt-dependent and independent FOXQ1 transcriptional output in colorectal cancers.

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