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Transcription profiling of human bronchial epithelial cells to low doses of cigarette smoke condensate - chronic exposure

ABSTRACT: BEAS-2B cells have been treated with low doses (20 ug/ml) of CSC for 4 months. As negative control BEAS-2B cells were treated with DMSO (the CSC solvent). Non-treated cells were cultivated in parallel. Experiment Overall Design: After each month total RNA was extracted from three replicates of CSC, DMSO and non-treated BEAS-2B cells and hybridized to Affymetrix GeneChips.

ORGANISM(S): Homo sapiens

SUBMITTER: Hubert Rehrauer

PROVIDER: E-GEOD-14383 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chronic exposure to cigarette smoke condensate in vitro induces epithelial to mesenchymal transition-like changes in human bronchial epithelial cells, BEAS-2B.

Veljkovic Emilija E Jiricny Josef J Menigatti Mirco M Rehrauer Hubert H Han Wanjiang W

Toxicology in vitro : an international journal published in association with BIBRA 20101121 2

Cigarette smoke causes lung tumorigenesis; however, the mechanisms underlying transformation are unknown. We investigated if tobacco compounds induce DNA promoter hypermethylation in BEAS-2B cells treated with low doses of cigarette smoke condensate (CSC) for one month. Transcriptional profiles and anchorage-independent growth were explored using Affymetrix microarray and soft agar assay, respectively. To investigate if tobacco compounds induce hypermethylation, CSC/dimethyl sulfoxide (DMSO)-tre ...[more]

PMID: 21095227

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