Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of expression in PAM765-transgenic vs GUS-transgenic Arabidopsis


ABSTRACT: PAM765 encodes the secretory protein of a phytopathogenic bacterium, Candidatus Phytoplasma asteris. To investigate the influence of PAM765 expression on the transcription profile of a host plant, we identified differences in the gene expression profiles between PAM765-transgenic and GUS-transgenic Arabidopsis plants using microarray analysis. Three independent transgenic lines were harvested from 2-week-old tengu- or GUS-transgenic plants, and were used for microarray analysis.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Shigetou Namba 

PROVIDER: E-GEOD-14564 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium.

Hoshi Ayaka A   Oshima Kenro K   Kakizawa Shigeyuki S   Ishii Yoshiko Y   Ozeki Johji J   Hashimoto Masayoshi M   Komatsu Ken K   Kagiwada Satoshi S   Yamaji Yasuyuki Y   Namba Shigetou S  

Proceedings of the National Academy of Sciences of the United States of America 20090327 15


One of the most important themes in agricultural science is the identification of virulence factors involved in plant disease. Here, we show that a single virulence factor, tengu-su inducer (TENGU), induces witches' broom and dwarfism and is a small secreted protein of the plant-pathogenic bacterium, phytoplasma. When tengu was expressed in Nicotiana benthamiana plants, these plants showed symptoms of witches' broom and dwarfism, which are typical of phytoplasma infection. Transgenic Arabidopsis  ...[more]

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