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Unknown,Transcriptomics,Genomics,Proteomics

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Comparisons of caruncles, intercaruncles, AI, IVF-ET and SCNT in pregnancies

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE14047: Comparison between caruncles and intercaruncles in AI, IVF-ET and SCNT pregnancies GSE14050: AI versus IVF-ET & AI versus SCNT Refer to individual Series

ORGANISM(S): Bos taurus

SUBMITTER: Olivier Sandra

PROVIDER: E-GEOD-14638 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Endometrium as an early sensor of in vitro embryo manipulation technologies.

Mansouri-Attia Nadéra N Sandra Olivier O Aubert Julie J Degrelle Séverine S Everts Robin E RE Giraud-Delville Corinne C Heyman Yvan Y Galio Laurent L Hue Isabelle I Yang Xiangzhong X Tian X Cindy XC Lewin Harris A HA Renard Jean-Paul JP

Proceedings of the National Academy of Sciences of the United States of America 20090318 14

Implantation is crucial for placental development that will subsequently impact fetal growth and pregnancy success with consequences on postnatal health. We postulated that the pattern of genes expressed by the endometrium when the embryo becomes attached to the mother uterus could account for the final outcome of a pregnancy. As a model, we used the bovine species where the embryo becomes progressively and permanently attached to the endometrium from day 20 of gestation onwards. At that stage, ...[more]

PMID: 19297625

Similar Datasets

Comparison between caruncles and intercaruncles in AI, IVF-ET and SCNT pregnancies

Project description:Implantation is crucial for placental development whose quality will directly impact fetal growth and pregnancy success with possible consequences on post-natal health. We postulated that early perturbations of the conceptus-maternal environment communication may alter the endometrium physiology that could account for the final reproductive outcome. Using cattle as an animal model, we compared gene expression profiles of the endometrial caruncular and intercaruncular areas at implantation in three types of pregnancies, namely artificial insemination (AI), in vitro fertilization with embryo transfer (IVF-ET) or somatic cell nuclear transfer (SCNT). Less than 35% of the differentially regulated genes were found to be common between AI, IVF-ET, and SCNT conditions. Compared to AI, numerous biological functions and several canonical pathways and genes were found to be significantly affected in IVF-ET or SCNT, with a major impact on metabolism and immune function in SCNT. Our data show that endometrium can fine-tune its physiology and could be considered as a biological sensor in response to pregnancy manipulations. Determining the limits of the endometrial plasticity should bring new insights on the contribution of the maternal compartment to the issue of pregnancy. Keywords: Fluorescence Microarray 30 samples

2010-06-20 | E-GEOD-14047 | biostudies-arrayexpress

AI versus IVF-ET & AI versus SCNT

Project description:Implantation is crucial for placental development which directly impacts fetal growth and pregnancy success with possible consequences on post-natal health. We postulated that early perturbations of the conceptus-maternal environment communication may alter the endometrium physiology that could account for the final reproductive outcome. Using cattle as an animal model, we compared gene expression profiles of the endometrial caruncular and intercaruncular areas during the critical period of implantation in three types of pregnancies: artificial insemination (AI), in vitro fertilization with embryo transfer (IVF-ET) or somatic cell nuclear transfer (SCNT). Less than 35% of the differentially expressed genes were found to be common between AI, IVF-ET, and SCNT conditions. Compared to AI, numerous biological functions and several canonical pathways and genes were found to be significantly affected in IVF-ET or SCNT, with a major impact on metabolism and immune function in SCNT. Our data show that the endometrium can fine-tune its physiology and could be considered as a biological sensor in response to pregnancy manipulations. Determining the limits of the endometrial plasticity should bring new insights on the contribution of the maternal compartment to the pregnancy outlet. Keywords: Fluorescence Microarray - Dye switch loop design 44 samples

2009-10-31 | E-GEOD-14050 | biostudies-arrayexpress

Comparison between caruncular and intercaruncular areas in the endometrium of cyclic and pregnant cows

Project description:At implantation the endometrium undergoes dramatic modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular [C] and the intercaruncular [IC] areas in cattle. The [C versus IC] direct comparison revealed 1177 and 453 DEG in cyclic and pregnant animals respectively, with a major impact of the conceptus on metabolism and cell adhesion. Keywords: Microarray fluorescence 10 Samples comparing caruncle and intercaruncle in pregnant cows, 10 Samples comparing caruncle and intercaruncle in cyclic cows.

2010-06-24 | E-GEOD-13728 | biostudies-arrayexpress

Cycle versus Pregnancy

Project description:At implantation the endometrium undergoes dramatic modifications necessary for its physical interactions with the trophoblast as well as the development of the conceptus. We aim to identify endometrial factors and pathways essential for a successful implantation in the caruncular [C] and the intercaruncular [IC] areas in cattle. Using a bovine oligo-array, expression profiles were established at day 20 of the estrous cycle or pregnancy (implantation) showing 446 and 1295 differentially expressed genes (DEG) in [C] and [IC] areas respectively. The impact of the conceptus was higher on the immune response function in [C] but more prominent on the regulation of metabolism function in [IC]. Keywords: Fluorescence Microarray 18 intercaruncular samples, 18 caruncular Samples. Comparisons of cycle (d20) and pregnancy (d20).

2010-06-24 | E-GEOD-13664 | biostudies-arrayexpress

Response of muscle to stress

Project description:As muscle function is relevant for the stress response, and as gene array and proteomics analyses have not been conducted to investigate muscle response in stress paradigm in birds, we developed generic approaches to assess molecular bases of muscle response to stress in chicken. Restraint test was chosen as stress situation because this treatment has previously been shown to be a high stressful situation in birds and mammals. Keywords: comparative genomic expression, skeletal muscle, restraint-transport stress, chicken 5 samples

2010-06-05 | E-GEOD-19644 | biostudies-arrayexpress

Transcriptome uterus

Project description:cDNA microarrays were used to identify the expression of the genes in uterus by comparison with two others regions of the oviduct not involved in the shell deposition. A total of 605 sequences specifically over-expressed were revealed. The expression of 15 of them was also confirmed using qRT-PCR, and confirmed the microarray expression. The 605 uterine transcripts corresponded to 469 and 437 different genes and proteins respectively. A global interpretation of their functions was assessed using the Gene Ontology terms and the most over-represented terms are relative to the mineral transport and transfer necessary to the calcification process. The study has revealed 53 proteins which could be secreted to be deposited in the shell to be biologically actives. The study of their functions reveals proteins known for their involvement in the biomineralization or with calcium binding properties to interact with the mineral phase during the eggshell fabric. A large group of proteins could be involved in the folding of the matrix, and a notable group of uterine secreted proteins was also identified as antibacterial to contribute to the antimicrobial protection of the egg. Finally the study revealed proteases and protease inhibitors which could play an active role in the regulation of the protein activities in the acellular uterine fluid where the eggshell is formed. Keywords: Laying hen, eggshell, oviduct, uterine expression, cDNA microarray, indirect cDNA labelling, Alexa Fluor dyes A balanced block hybridization design (Dye switch) was used where half of the samples were labelled with AlexaÂ® 555 fluorescent dye and the other half with AlexaÂ® 647. A total of 16 microarray slides were used for hybridization to 32 samples that correspond to four tissue contrast (uterus versus magnum and uterus versus white isthmus).

2010-05-06 | E-GEOD-17267 | biostudies-arrayexpress

Gene expression profile analysis of Fat and Lean chickens

Project description:The aim of this study was to identify genes involved in the variation of the muscle glycogen content at death (estimated through the glycolytic potential, GP), a determining factor of meat quality in chicken. Gene expression profiles of Pectoralis major muscle were established using microarrays. We compared Fat and Lean chickens issued from two lines divergently selected for abdominal fatness and also differed for muscle GP. A total of 197 genes were differentially expressed between Fat and Lean pure chickens. Several of these genes were validated by qRT-PCR. For the genes with human orthologs, annotation analyses were performed and mainly revealed pathways involved carbohydrate, fatty-acid, and protein metabolism. The relationship between gene expression and meat quality has to now be validated by further e-QTL studies on the F2 population. 8 samples from Fat chickens were compared to 8 samples from Lean chickens, 4 of these were dye-swapped.

2010-06-24 | E-GEOD-17445 | biostudies-arrayexpress

Gene expression profile analysis of F2 cross between Fat and Lean chickens

Project description:The aim of this study was to identify genes involved in the variation of the muscle glycogen content at death (estimated through the glycolytic potential, GP), a determining factor in the meat quality of chicken. The gene expression profiles of the Pectoralis major muscle were established using microarrays. We compared animals showing extreme GP values, issued from a F2 cross between Fat and Lean lines. A total of 254 genes were differentially expressed between F2 birds with low or high GP. Several of these genes were validated by qRT-PCR. For the genes with human orthologs, annotation analyses were performed and mainly revealed pathways involved in carbohydrate, fatty-acid and protein metabolism. The relationship between gene expression and meat quality has to now be validated by further e-QTL studies on the F2 population. 8 high GP samples compared to 8 low GP samples, 4 of these were dye-swapped.

2010-06-24 | E-GEOD-17428 | biostudies-arrayexpress

Postnatal response of the intestine in 19 d-old IUGR piglets

Project description:Comparison of distal small intestine and colon in IntraUterine Growth Restricted versus normal birth weight piglets at the age of 19 days of life. Keywords: Nutritional state comparative analysis. 22 samples - One sample versus a pool

2010-05-16 | E-GEOD-14716 | biostudies-arrayexpress

Postnatal intestinal response of IUGR piglets to high proteins before weaning

Project description:Comparison of distal small intestine in IntraUterine Growth Restricted piglets fed either adequate or high protein artificial milks during suckling period. Comparison at 7 and 28 days of life. Keywords: Nutritional response 22 samples - One sample versus a pool

2010-05-16 | E-GEOD-18249 | biostudies-arrayexpress

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