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Bacillus subtilis: Smc-TAP ChIP-on-chip

ABSTRACT: Genome-wide analysis of Smc distribution using ChIP-on-chip ChIP DNA versus Input DNA Keywords: ChIP-chip Combined ChIP and Input DNA from three independent experiments on single array

ORGANISM(S): Bacillus subtilis

SUBMITTER: Stephan Gruber

PROVIDER: E-GEOD-14693 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Recruitment of condensin to replication origin regions by ParB/SpoOJ promotes chromosome segregation in B. subtilis.

Gruber Stephan S Errington Jeff J

Cell 20090501 4

Proper segregation of DNA replication products is essential in all cells. In Bacillus subtilis, two protein complexes have been implicated in this process: the ParAB homologs, Soj and Spo0J, and the bacterial Smc/ScpAB complex, also called condensin. Here we demonstrate that Smc is highly enriched in the region around the origin of replication, specifically near parS sites to which Spo0J binds and at highly transcribed genes. Furthermore, we find that efficient recruitment of Smc to a large regi ...[more]

PMID: 19450516

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Bacillus subtilis: Smc-TAP ChIP-on-chip

Project description:Genome-wide analysis of Smc distribution using ChIP-on-chip ChIP DNA versus Input DNA Keywords: ChIP-chip

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A low Smc flux avoids collisions and facilitates chromosome organization in B. subtilis

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