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Gene expression changes by 2,4-dinitrochlorobenzene in human bulge-derived keratinocytes

ABSTRACT: In the epidermis, keratinocytes are involved in physical and first-line immune protection of the host. In this work, we analyzed molecular responses after the addition of contact sensitizers, 2,4-dinitrochlorobenzene (DNCB) using cultured human keratinocytes from the bulge region of a plucked hair follicle (bulge-derived keratinocytes; BDKs) in comparison with neonatal human epidermal keratinocytes NHEK and human monocytic leukemia THP-1. The changes in gene expression by the treatment of DNCB in BDKs were different from those in THP-1. Many genes orchestrating keratinocyte differentiation, which interact TGF-Î² and BMP signaling pathway, were significantly up-regulated in response to DNCB. Keywords: Comparison analysis of gene expression changes by the treatment of DNCB three cell strains composed of four samples; two BDKs established from different donors, NHEK, and THP-1. One replicate per array.

ORGANISM(S): Homo sapiens

SUBMITTER: Tomoko Hashimoto-Tamaoki

PROVIDER: E-GEOD-14838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profiling of genes expressed in cultured human keratinocytes established from the bulge region of hair follicles.

Project description:We established a culture method of human keratinocytes from the bulge region of a plucked hair follicle, that contains multipotent epithelial stem cells with high proliferative potential. Using our method, keratinocyte cultures were successfully obtained from all subjects without invasive skin biopsies. We compared the gene expression profiles between the cultured keratinocytes derived from human hair-follicle-bulge (bulgeM-bM-^@M-^Sderived keratinocytes; BDKs) and neonatal human epidermal keratinocytes (NHEKs), and between BDKs from donors with atopic dermatitis and non-atopic controls using microarray analysis. Keywords: expressin profiling Two cell cultures, BDK vs. NHEK cells. 18 BDKs; derived from eighteen healthy volunteers , 6 NHEKs; purchased from Kurabo (Osaka, Japan). One replicate per array.

2013-02-05 | E-GEOD-13709 | biostudies-arrayexpress

Gene expression changes by 2,4-dinitrochlorobenzene in human bulge-derived keratinocytes

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Genome-wide binding of WDR5 and GRHL3

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2012-11-10 | E-GEOD-42180 | biostudies-arrayexpress

Expression and ChIP-seq analysis LPS stimulated THP-1 cells

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