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SOX2 overexpression effect on human lung squamous cells

ABSTRACT: We have identified SOX2 as a new oncogene and a likely driver of recurrent 3q26.3 amplifications in lung Squamous Cell Carcinoma. SOX2 is a crucial transcription factor implicated in Embryonic and Neural Stem Cells, that we found widely activatd in human lung SCC. This part of the study aimed at analyzing the transcriptomic consequences of SOX2 overexpression in a simple in vitro model (human lung squamous immortalized cells). Human lung squamous BEAS-2B cells were transduced for Control or SOX2 expression, and their transcriptomes compared using Affymetrix arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Hussenet

PROVIDER: E-GEOD-14883 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SOX2 is an oncogene activated by recurrent 3q26.3 amplifications in human lung squamous cell carcinomas.

Hussenet Thomas T Dali Soraya S Exinger Julien J Monga Ben B Jost Bernard B Dembelé Doulaye D Martinet Nadine N Thibault Christelle C Huelsken Joerg J Brambilla Elisabeth E du Manoir Stanislas S

PloS one 20100129 1

Squamous cell carcinoma (SCC) of the lung is a frequent and aggressive cancer type. Gene amplifications, a known activating mechanism of oncogenes, target the 3q26-qter region as one of the most frequently gained/amplified genomic sites in SCC of various types. Here, we used array comparative genomic hybridization to delineate the consensus region of 3q26.3 amplifications in lung SCC. Recurrent amplifications occur in 20% of lung SCC (136 tumors in total) and map to a core region of 2 Mb (Megaba ...[more]

PMID: 20126410

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