Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

C. elegans embryos: control vs. mes-2 mutant

ABSTRACT: Transcriptional profiling of early C. elegans embryos comparing control (N2) embryos with mes-2 mutant embryos at different developmental stages: 2E (24-40 cells), 4E (50-90 cells) and 8E stage (100-200 cells). Goal was to determine the effects of mes-2 loss on global gene expression as embryos transit from a developmentally plastic state (2E stage) to the onset of differentiation (8E stage). Our microarray data showed that early-expressed genes remain active, differentiation genes fail to reach wild-type levels in mes-2 mutant embryos at the 8E stage. Two-condition experiment, wild type vs. mes-2 embryos. Biological replicates: 3 control replicates, 3 mes-2 replicates for each stage.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Tatiana Yuzyuk

PROVIDER: E-GEOD-14913 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The polycomb complex protein mes-2/E(z) promotes the transition from developmental plasticity to differentiation in C. elegans embryos.

Yuzyuk T T Fakhouri T H I TH Kiefer J J Mango S E SE

Developmental cell 20090501 5

We have used expression profiling and in vivo imaging to characterize Caenorhabditis elegans embryos as they transit from a developmentally plastic state to the onset of differentiation. Normally, this transition is accompanied by activation of developmental regulators and differentiation genes, downregulation of early-expressed genes, and large-scale reorganization of chromatin. We find that loss of plasticity and differentiation onset depends on the Polycomb complex protein mes-2/E(Z). mes-2 m ...[more]

PMID: 19460346

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Dataset Information

C. elegans embryos: control vs. mes-2 mutant

Publications

The polycomb complex protein mes-2/E(z) promotes the transition from developmental plasticity to differentiation in C. elegans embryos.

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