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Consequences of GATA1 expression in Gata1- G1ME Murine Megakaryocyte/Erythrocyte Progenitor Cell Line

ABSTRACT: G1ME cells are GATA1-deficient murine bipotential megakaryocyte/erythrocyte progenitor cells derived from Gata1-negative murine ES cells. In order to assess the impact of GATA1 on gene regulation and cell differentiation, an expression construct was used to transiently produce high levels of GATA1. Cells transduced with this construct or a vector control were harvested at 18 and 42 hours, and gene expression was analyzed using Affymetrix MOE430 version 2 arrays. Both vector control and GATA1-expressing cells were isolated by FACS for GFP and 18 and 42 hours. Biologic triplicates were performed for each construct at each timepoint.

ORGANISM(S): Mus musculus

SUBMITTER: Charles Bailey

PROVIDER: E-GEOD-14980 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate.

Chou Stella T ST Khandros Eugene E Bailey L Charles LC Nichols Kim E KE Vakoc Christopher R CR Yao Yu Y Huang Zan Z Crispino John D JD Hardison Ross C RC Blobel Gerd A GA Weiss Mitchell J MJ

Blood 20090602 5

GATA-1 and PU.1 are essential hematopoietic transcription factors that control erythromegakaryocytic and myelolymphoid differentiation, respectively. These proteins antagonize each other through direct physical interaction to repress alternate lineage programs. We used immortalized Gata1(-) erythromegakaryocytic progenitor cells to study how PU.1/Sfpi1 expression is regulated by GATA-1 and GATA-2, a related factor that is normally expressed at earlier stages of hematopoiesis. Both GATA factors b ...[more]

PMID: 19491391

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Project description:G1ME cells are GATA1-deficient murine bipotential megakaryocyte/erythrocyte progenitor cells derived from Gata1-negative murine ES cells. In order to assess the impact of GATA1 on gene regulation and cell differentiation, an expression construct was used to transiently produce high levels of GATA1. Cells transduced with this construct or a vector control were harvested at 18 and 42 hours, and gene expression was analyzed using Affymetrix MOE430 version 2 arrays.

2009-06-10 | GSE14980 | GEO

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