Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in Phytophthora sojae mycelia, germinating zoospores, and during infection of soybean hypocotyls


ABSTRACT: Total RNA extracted from Phytophthora sojae (strain P6497) and infected soybean hypocotyls (cultivar Harosoy) provided template for synthesis of cDNA probes used in the microarray hybridizations. Infected plant hypocotyls were sampled 6 h, 12 h, 24 h, and 48 h after inoculation. Mycelia were grown on synthetic media (H&S) or vegetable juice media (V8). Zoospores were sampled at 0 h, 2 h and 6 h after inducing encystment and germination by agitation. We used microarrays to characterize gene expression patterns in the root rot pathogen Phytophthora sojae and its host Glycine max. Keywords: infection time course, zoospore germination time course, media formulation response 28 samples from 9 treatments; 2 to 5 biological replicates per treatment.

ORGANISM(S): Phytophthora sojae

SUBMITTER: Mark Gijzen 

PROVIDER: E-GEOD-15100 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Phytophthora sojae avirulence locus Avr3c encodes a multi-copy RXLR effector with sequence polymorphisms among pathogen strains.

Dong Suomeng S   Qutob Dinah D   Tedman-Jones Jennifer J   Kuflu Kuflom K   Wang Yuanchao Y   Tyler Brett M BM   Gijzen Mark M  

PloS one 20090515 5


Root and stem rot disease of soybean is caused by the oomycete Phytophthora sojae. The avirulence (Avr) genes of P. sojae control race-cultivar compatibility. In this study, we identify the P. sojae Avr3c gene and show that it encodes a predicted RXLR effector protein of 220 amino acids. Sequence and transcriptional data were compared for predicted RXLR effectors occurring in the vicinity of Avr4/6, as genetic linkage of Avr3c and Avr4/6 was previously suggested. Mapping of DNA markers in a F(2)  ...[more]

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