Unknown,Transcriptomics,Genomics,Proteomics

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Shg1p associated RNAs

ABSTRACT: RNA co-immunopurification with TAP-tagged Shg1p (a component of the Set1 histone methyltransferase complex, SET1C) from Saccharomyces cerevisiae. An untagged (BY4741) served as a control (Gerber et al. 2004). Cells were grown to mid-log phase in rich media and harvested by centrifugation. TAP-tagged Shg1 was affinity-purified from cell-free extracts with IgG sepharose and eluted with TEV protease. RNA was isolated from extract (=input) and from the purified protein sample with the RNAeasy Kit (Qiagen). RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast oligo microarrays over night at 42 degrees in formamide-based hybridization buffer. For the method see experimental procedure for RNA IP (Gerber/Dichtl). An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andre Gerber

PROVIDER: E-GEOD-15247 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cotranslational assembly of the yeast SET1C histone methyltransferase complex.

Halbach André A Zhang Haidi H Wengi Agnieszka A Jablonska Zofia Z Gruber Isabel M L IM Halbeisen Regula E RE Dehé Pierre-Marie PM Kemmeren Patrick P Holstege Frank F Géli Vincent V Gerber André P AP Dichtl Bernhard B

The EMBO journal 20090827 19

While probing the role of RNA for the function of SET1C/COMPASS histone methyltransferase, we identified SET1RC (SET1 mRNA-associated complex), a complex that contains SET1 mRNA and Set1, Swd1, Spp1 and Shg1, four of the eight polypeptides that constitute SET1C. Characterization of SET1RC showed that SET1 mRNA binding did not require associated Swd1, Spp1 and Shg1 proteins or RNA recognition motifs present in Set1. RNA binding was not observed when Set1 protein and SET1 mRNA were derived from in ...[more]

PMID: 19713935

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Dataset Information

Shg1p associated RNAs

Publications

Cotranslational assembly of the yeast SET1C histone methyltransferase complex.

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