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Profiling of FACs sorted subpopulations of iPS cells from cell surface marker profile

ABSTRACT: Two independent induced pluripotent stem cell lines: ES4CL1 (derived from foreskin) and MR90C2 (derived from lung Fibroblast) were maintained on inactivated mouse embryonic fibroblast (MEF) feeder cells in the presence of ESC media containing DMEM, 20% knock-out serum replacement with 100ng/mL of bFGF. 7 days after seeding, cells were harvested in TrypLE Express and FACs sorted using antibodies detecting the presence of cell surface antigens GCTM-2 and CD9. 4 independent fractions were isolated after cell sorting: P4 (CD9-Negative, GCTM-2 Negative); P5 (CD9-Low, GCTM-2 Low); P6 (CD9-Medium, GCTM-2 medium) and P7 (CD9-High, GCTM-2 High). Each individual experiment was performed in triplicate. Keywords: cell type comparison Three consecutive passages of both ES4CL1 and MR90C2 cells were grown in standard conditions, FACs sorted cells collected, and total RNA isolated. Microarray was performed on a total of 24 samples, (3 replicates of 4 FACs sorted populations from two independent cell lines).

ORGANISM(S): Homo sapiens

SUBMITTER: Sean Grimmond 

PROVIDER: E-GEOD-15283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of unsafe human induced pluripotent stem cell lines using a robust surrogate assay for pluripotency.

Polanco Juan Carlos JC   Ho Mirabelle S H MS   Wang Bei B   Zhou Qi Q   Wolvetang Ernst E   Mason Elizabeth E   Wells Christine A CA   Kolle Gabriel G   Grimmond Sean M SM   Bertoncello Ivan I   O'Brien Carmel C   Laslett Andrew L AL  

Stem cells (Dayton, Ohio) 20130801 8

Human induced pluripotent stem cells (hiPSC) have the potential to generate healthy cells and tissues for the study and medical treatment of a large number of diseases. The utility of putative hiPSC-based therapies is constrained by a lack of robust quality-control assays that address the stability of the cells or their capacity to form teratomas after differentiation. Here we report that virally derived hiPSC, but not human embryonic stem cells (hESC) or hiPSC derived using episomal nonintegrat  ...[more]

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