Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

ESC (HES2, MEL1 or H9) grown in various conditions and FACs sorted for GCTM-2 and CD9

ABSTRACT: In the following experiment, three different hESC cell lines (HES2, MEL1 and H9) were grown in the presence of KOSR, KOSR or mTESR containing media respectively. KOSR (Knockout serum replacement medium) is a standard media allowing the growth of hESC without the need for manual passaging - Enzymatic passaging is used instread. mTESR (Ludwig et al., 2007) is a media allowing the growth of hESC on matrigel with enzymatic passaging. At day 7 after passaging, these cells were FACs sorted for the presence of GCTM-2 and CD9 into 4 distinct fractions (p4: GCTM-2-neg, CD9-neg; p5: GCTM-2-low, CD9-low; p6: GCTM-2-medium, CD9-medium and p7: GCTM-2-high, CD9-high). For each cell line-subfraction combination, RNA was harvested and subject to microarray. From each experiment (individual cell line), 4 samples were collected (p4, p5, p6 and p7) and these were subject to microarray. In each case the experiment was performed in triplicate Three independent experiments of three consecutive passages of ESC cells were grown and subject to FACs sorting, collection and microarray. A total of 36 samples, (3 experiments of 3 replicates of 4 sorted populations of cells (p4, p5, p6, p7))

ORGANISM(S): Homo sapiens

SUBMITTER: Gabriel Kolle

PROVIDER: E-GEOD-13877 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling.

Kolle Gabriel G Ho Mirabelle M Zhou Qi Q Chy Hun S HS Krishnan Keerthana K Cloonan Nicole N Bertoncello Ivan I Laslett Andrew L AL Grimmond Sean M SM

Stem cells (Dayton, Ohio) 20091001 10

Surface marker expression forms the basis for characterization and isolation of human embryonic stem cells (hESCs). Currently, there are few well-defined protein epitopes that definitively mark hESCs. Here we combine immunotranscriptional profiling of hESC lines with membrane-polysome translation state array analysis (TSAA) to determine the full set of genes encoding potential hESC surface marker proteins. Three independently isolated hESC lines (HES2, H9, and MEL1) grown under feeder and feeder ...[more]

PMID: 19650036

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Project description:SBML model exported from PottersWheel on 2007-09-19 15:35:47. The values for parameters and the inital concentrations of this model where directly provided by the main author: Parameter values parameter value unit p1 0.0025 1/min p2 0.0784 1/min p3 0.0013 1/min p4 0.0827 1/min p5 0.0091 1/min p6 0.000064 1/(nmole*min) p7 0.0397 1/min p8 1000 nmole p9 0.0098 1/(nmole*min) p10 1.6 1/min p11 1000 nmole p12 0.0003 ml/min The basal chamber volume was taken as 1 ml, the apical as 1.5. As starting values x1 was set to 88 nmole, all other species to 0. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

Dataset Information

ESC (HES2, MEL1 or H9) grown in various conditions and FACs sorted for GCTM-2 and CD9

Publications

Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling.

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