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Cells following mtDNA loss, or NAR1 repression

ABSTRACT: Expression analysis of cells the given amount of time after mtDNA was lost (or Nar1 expression was repressed) compared to pretreatment (or NAR1 being fully expressed). One time course experiment (Cells a given amount of time following mtDNA loss compared to cells with intact mtDNA), with 2 two condition experiments (Cells with the ATP1-111 genotype 27 hours following mtDNA loss compared to the same cells with intact mtDNA, and cells 27 hours following repression of NAR1 comared to cells expressing NAR1). Each data point had 3 biological replicates, and was dye-swapped. One replicate per array.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Joshua Veatch

PROVIDER: E-GEOD-15302 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mitochondrial dysfunction leads to nuclear genome instability via an iron-sulfur cluster defect.

Veatch Joshua R JR McMurray Michael A MA Nelson Zara W ZW Gottschling Daniel E DE

Cell 20090601 7

Mutations and deletions in the mitochondrial genome (mtDNA), as well as instability of the nuclear genome, are involved in multiple human diseases. Here, we report that in Saccharomyces cerevisiae, loss of mtDNA leads to nuclear genome instability, through a process of cell-cycle arrest and selection we define as a cellular crisis. This crisis is not mediated by the absence of respiration, but instead correlates with a reduction in the mitochondrial membrane potential. Analysis of cells undergoi ...[more]

PMID: 19563757

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