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Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunoprecipitation to measure RNA polymerase II occupancy in fission yeast

ABSTRACT: The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Daniel Lackner

PROVIDER: E-TABM-219 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A network of multiple regulatory layers shapes gene expression in fission yeast.

Lackner Daniel H DH Beilharz Traude H TH Marguerat Samuel S Mata Juan J Watt Stephen S Schubert Falk F Preiss Thomas T Bähler Jürg J

Molecular cell 20070401 1

Gene expression is controlled at multiple layers, and cells may integrate different regulatory steps for coherent production of proper protein levels. We applied various microarray-based approaches to determine key gene-expression intermediates in exponentially growing fission yeast, providing genome-wide data for translational profiles, mRNA steady-state levels, polyadenylation profiles, start-codon sequence context, mRNA half-lives, and RNA polymerase II occupancy. We uncovered widespread and ...[more]

PMID: 17434133

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